Single frequency impedimetric immunosensor for atrazine detection

Ángel Rodríguez, Enrique Valera, Javier Ramón-Azcón, F. J. Sanchez, M. P. Marco, Luis M. Castañer

Research output: Contribution to journalArticlepeer-review


A novel impedimetric immunosensor for the detection of a wide variety of compounds, based on a two coplanar non-passivated interdigitated metallic μ-electrodes and non-labeled immunoreactives, has been designed and developed. A very useful detection method is the impedance spectroscopy sweeping a wide frequency range. This is a powerful, but cumbersome tool to study the sensor performance providing trustworthy results. In this work, it is shown that the exploitation of the changes observed of the impedance at a single frequency provide very good correlation with antibody concentration and hence greatly reduces the signal acquisition and processing complexity. It has thereby a great potential for low cost, low power, fast response and simple use for the in-field or at-line applications. In this work, the application of the method to atrazine detection is described. The results show that the immunosensor signal is a function of the pesticide concentration following a competitive binding relationship, with limits of detection lower than the maximum residue level required by EC for atrazine in wine grapes and other foodstuff products.

Original languageEnglish (US)
Pages (from-to)921-928
Number of pages8
JournalSensors and Actuators, B: Chemical
Issue number2
StatePublished - Feb 22 2008
Externally publishedYes


  • Atrazine
  • Immunosensor
  • Impedance spectroscopy
  • Interdigitated μ-electrodes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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