Single chain epidermal growth factor receptor antibody conjugated nanoparticles for in vivo tumor targeting and imaging

Lily Yang, Hui Mao, Y. Andrew Wang, Zehong Cao, Xianghong Peng, Xiaoxia Wang, Hongwei Duan, Chunchun Ni, Qingan Yuan, Gregory Adams, Mark Q. Smith, William C. Wood, Xiaohu Gao, Shuming Nie

Research output: Contribution to journalArticlepeer-review

Abstract

Epidermal growth factor receptor (EGFR) targeted nanoparticle are developed by conjugating a single-chain anti-EGFR antibody (ScFvEGFR) to surface functionalized quantum dots (QDs) or magnetic iron oxide (10) nanoparticles. The results show that ScFvEGFR can be successfully conjugated to the nanoparticles, resulting in compact ScFvEGFR nanoparticles that specifically bind to and are internalized by EGFR-expressing cancer cells, thereby producing a fluorescent signal or magnetic resonance imaging (MRI) contrast. In vivo tumor targeting and uptake of the nanoparticles in human cancer cells is demonstrated after systemic delivery of ScFvEGFR-QDs or ScFvEGFR-IO nanoparticles into an orthotopic pancreatic cancer model. Therefore, ScFvEGFR nanoparticles have potential to be used as a molecular-targeted in vivo tumor imaging agent. Efficient internalization of ScFvEGFR nanoparticles into tumor cells after systemic delivery suggests that the EGFR-targeted nanoparticles can also be used for the targeted delivery of therapeutic agents.

Original languageEnglish (US)
Pages (from-to)235-243
Number of pages9
JournalSmall
Volume5
Issue number2
DOIs
StatePublished - Jan 19 2009
Externally publishedYes

Keywords

  • Antibodies
  • Drug delivery
  • Nanoparticles
  • Proteins
  • Quantum dots

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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