Single-cell photoacoustic thermometry

Liang Gao; Lidai Wang; Chiye Li; Yan Liu; Haixin Ke; Chi Zhang; Lihong V. Wang

doi:10.1117/1.JBO.18.2.026003

Single-cell photoacoustic thermometry

Liang Gao, Lidai Wang, Chiye Li, Yan Liu, Haixin Ke, Chi Zhang, Lihong V. Wang

Research output: Contribution to journal › Article › peer-review

Abstract

A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.

Original language	English (US)
Article number	026003
Journal	Journal of biomedical optics
Volume	18
Issue number	2
DOIs	https://doi.org/10.1117/1.JBO.18.2.026003
State	Published - Feb 2013
Externally published	Yes

Keywords

Photoacoustic microscopy
Single-cell thermometry
cell imaging

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomedical Engineering
Biomaterials

Online availability

10.1117/1.JBO.18.2.026003

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title = "Single-cell photoacoustic thermometry",

abstract = "A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.",

keywords = "Photoacoustic microscopy, Single-cell thermometry, cell imaging",

author = "Liang Gao and Lidai Wang and Chiye Li and Yan Liu and Haixin Ke and Chi Zhang and Wang, {Lihong V.}",

note = "Funding Information: This work was sponsored by the National Institutes of Health (NIH) under grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, and R01 CA159959. L. V. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc.; however, neither provided support for this work.",

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doi = "10.1117/1.JBO.18.2.026003",

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volume = "18",

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AU - Gao, Liang

AU - Wang, Lidai

AU - Li, Chiye

AU - Liu, Yan

AU - Ke, Haixin

AU - Zhang, Chi

AU - Wang, Lihong V.

N1 - Funding Information: This work was sponsored by the National Institutes of Health (NIH) under grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, and R01 CA159959. L. V. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc.; however, neither provided support for this work.

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Y1 - 2013/2

N2 - A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.

AB - A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.

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KW - cell imaging

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Single-cell photoacoustic thermometry

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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