Single-cell photoacoustic thermometry

Liang Gao, Lidai Wang, Chiye Li, Yan Liu, Haixin Ke, Chi Zhang, Lihong V. Wang

Research output: Contribution to journalArticle

Abstract

A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.

Original languageEnglish (US)
Article number026003
JournalJournal of biomedical optics
Volume18
Issue number2
DOIs
StatePublished - Feb 1 2013

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Keywords

  • Photoacoustic microscopy
  • Single-cell thermometry
  • cell imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Gao, L., Wang, L., Li, C., Liu, Y., Ke, H., Zhang, C., & Wang, L. V. (2013). Single-cell photoacoustic thermometry. Journal of biomedical optics, 18(2), [026003]. https://doi.org/10.1117/1.JBO.18.2.026003