TY - JOUR
T1 - Single-cell MALDI
T2 - A new tool for direct peptide profiling
AU - Li, Lingjun
AU - Garden, Rebecca W.
AU - Sweedler, Jonathan V.
N1 - Funding Information:
This study is supported by the National Institutes of Health through grant NS 31609 and the National Science Foundation with CHE 98-77071.
PY - 2000/4/1
Y1 - 2000/4/1
N2 - Matrix-assisted laser desorption-ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical approach that is well suited for obtaining molecular weights of peptides and proteins from complex samples. MALDI-MS can profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation, except for the cell isolation and matrix application. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing as well as the direct spatial mapping of peptides in cells and tissues are discussed. Copyright (C) 2000 Elsevier Science Ltd.
AB - Matrix-assisted laser desorption-ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical approach that is well suited for obtaining molecular weights of peptides and proteins from complex samples. MALDI-MS can profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation, except for the cell isolation and matrix application. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing as well as the direct spatial mapping of peptides in cells and tissues are discussed. Copyright (C) 2000 Elsevier Science Ltd.
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U2 - 10.1016/S0167-7799(00)01427-X
DO - 10.1016/S0167-7799(00)01427-X
M3 - Short survey
C2 - 10740261
AN - SCOPUS:0034176654
SN - 0167-7799
VL - 18
SP - 151
EP - 160
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 4
ER -