Abstract

Matrix-assisted laser desorption-ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical approach that is well suited for obtaining molecular weights of peptides and proteins from complex samples. MALDI-MS can profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation, except for the cell isolation and matrix application. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing as well as the direct spatial mapping of peptides in cells and tissues are discussed. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)151-160
Number of pages10
JournalTrends in Biotechnology
Volume18
Issue number4
DOIs
StatePublished - Apr 1 2000

Fingerprint

Peptides
Ionization
Desorption
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Lasers
Mass spectrometry
Peptide Mapping
Cell Separation
Tissue
Proteins
Post Translational Protein Processing
Molecular Weight
Technology
Molecular weight

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Single-cell MALDI : A new tool for direct peptide profiling. / Li, Lingjun; Garden, Rebecca W.; Sweedler, Jonathan V.

In: Trends in Biotechnology, Vol. 18, No. 4, 01.04.2000, p. 151-160.

Research output: Contribution to journalShort survey

Li, Lingjun ; Garden, Rebecca W. ; Sweedler, Jonathan V. / Single-cell MALDI : A new tool for direct peptide profiling. In: Trends in Biotechnology. 2000 ; Vol. 18, No. 4. pp. 151-160.
@article{ed813b76f0e448a3a79d24cc705bc857,
title = "Single-cell MALDI: A new tool for direct peptide profiling",
abstract = "Matrix-assisted laser desorption-ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical approach that is well suited for obtaining molecular weights of peptides and proteins from complex samples. MALDI-MS can profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation, except for the cell isolation and matrix application. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing as well as the direct spatial mapping of peptides in cells and tissues are discussed. Copyright (C) 2000 Elsevier Science Ltd.",
author = "Lingjun Li and Garden, {Rebecca W.} and Sweedler, {Jonathan V}",
year = "2000",
month = "4",
day = "1",
doi = "10.1016/S0167-7799(00)01427-X",
language = "English (US)",
volume = "18",
pages = "151--160",
journal = "Trends in Biotechnology",
issn = "0167-7799",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - Single-cell MALDI

T2 - A new tool for direct peptide profiling

AU - Li, Lingjun

AU - Garden, Rebecca W.

AU - Sweedler, Jonathan V

PY - 2000/4/1

Y1 - 2000/4/1

N2 - Matrix-assisted laser desorption-ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical approach that is well suited for obtaining molecular weights of peptides and proteins from complex samples. MALDI-MS can profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation, except for the cell isolation and matrix application. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing as well as the direct spatial mapping of peptides in cells and tissues are discussed. Copyright (C) 2000 Elsevier Science Ltd.

AB - Matrix-assisted laser desorption-ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical approach that is well suited for obtaining molecular weights of peptides and proteins from complex samples. MALDI-MS can profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation, except for the cell isolation and matrix application. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing as well as the direct spatial mapping of peptides in cells and tissues are discussed. Copyright (C) 2000 Elsevier Science Ltd.

UR - http://www.scopus.com/inward/record.url?scp=0034176654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034176654&partnerID=8YFLogxK

U2 - 10.1016/S0167-7799(00)01427-X

DO - 10.1016/S0167-7799(00)01427-X

M3 - Short survey

C2 - 10740261

AN - SCOPUS:0034176654

VL - 18

SP - 151

EP - 160

JO - Trends in Biotechnology

JF - Trends in Biotechnology

SN - 0167-7799

IS - 4

ER -