@article{73ba209c225f411db7c8ce4fe601534d,
title = "Single Cell Analysis of Proteoforms",
abstract = "We introduce single cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells dropcast onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enable a scalable approach to single cell proteomics. This new scPiMS platform addresses the throughput bottleneck in single cell proteomics and boosts the cell processing rate by several fold while accessing protein composition with higher coverage.",
keywords = "individual ion mass spectrometry, mass spectrometry, nano-DESI, proteoform assignment score, single cell, top-down proteomics",
author = "Pei Su and Hollas, {Michael A.R.} and Butun, {Fatma Ayaloglu} and Kanchustambham, {Vijaya Lakshmi} and Stanislav Rubakhin and Namrata Ramani and Greer, {Joseph B.} and Early, {Bryan P.} and Fellers, {Ryan T.} and Caldwell, {Michael A.} and Sweedler, {Jonathan V.} and Kafader, {Jared O.} and Kelleher, {Neil L.}",
note = "The authors also thank Prof. Chad Mirkin (Northwestern University) for valuable discussions. High-contrast bright field imaging experiments were performed with the help from Biological Imaging Facility at Northwestern University (RRID:SCR_017767), graciously supported by the Chemistry for Life Processes Institute, the NU Office for Research, the Department of Molecular Biosciences and the Rice Foundation. Funding from National Institutes of Health P41 GM108569 (N.L.K.), National Institutes of Health UH3 CA246635 (N.L.K.), National Institutes of Health P30 DA018310 (N.L.K. and J.V.S.), and National Institutes of Health P30 CA060553 (awarded to the Robert H. Lurie Comprehensive Cancer Center).",
year = "2024",
month = jun,
day = "7",
doi = "10.1021/acs.jproteome.4c00075",
language = "English (US)",
volume = "23",
pages = "1883--1893",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "6",
}