Single Cell Analysis of Proteoforms

Pei Su, Michael A.R. Hollas, Fatma Ayaloglu Butun, Vijaya Lakshmi Kanchustambham, Stanislav Rubakhin, Namrata Ramani, Joseph B. Greer, Bryan P. Early, Ryan T. Fellers, Michael A. Caldwell, Jonathan V. Sweedler, Jared O. Kafader, Neil L. Kelleher

Research output: Contribution to journalArticlepeer-review

Abstract

We introduce single cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells dropcast onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enable a scalable approach to single cell proteomics. This new scPiMS platform addresses the throughput bottleneck in single cell proteomics and boosts the cell processing rate by several fold while accessing protein composition with higher coverage.

Original languageEnglish (US)
JournalJournal of Proteome Research
DOIs
StateAccepted/In press - 2024

Keywords

  • individual ion mass spectrometry
  • mass spectrometry
  • nano-DESI
  • proteoform assignment score
  • single cell
  • top-down proteomics

ASJC Scopus subject areas

  • General Chemistry
  • Biochemistry

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