@article{c06e9ae30b514bf09f380bbd1a89ed23,
title = "Simultaneous label-free autofluorescence and multi-harmonic imaging reveals in vivo structural and metabolic changes in murine skin",
abstract = "Simultaneous quantification of multifarious cellular metabolites and the extracellular matrix in vivo has been long sought. Simultaneous label-free autofluorescence and multi-harmonic (SLAM) microscopy has achieved simultaneous four-channel nonlinear imaging to study tissue structure and metabolism. In this study, we implemented two laser systems and directly compared SLAM microscopy with conventional two-photon microscopy for in vivo imaging. We found that three-photon imaging of adenine dinucleotide (phosphate) (NAD(P)H) in SLAM microscopy using our tailored laser source provided better resolution, contrast, and background suppression than conventional two-photon imaging of NAD(P)H. We also integrated fluorescence lifetime imaging with SLAM microscopy, and enabled differentiation of free and bound NAD(P)H. We imaged murine skin in vivo and showed that changes in tissue structure, cell dynamics, and metabolism can be monitored simultaneously in real-time. We also discovered an increase in metabolism and protein-bound NAD(P)H in skin cells during the early stages of wound healing.",
author = "Lee, {Jang Hyuk} and Rico-Jimenez, {Jose J.} and Chi Zhang and Aneesh Alex and Chaney, {Eric J.} and Ronit Barkalifa and Spillman, {Darold R.} and Marina Marjanovic and Zane Arp and Hood, {Steve R.} and Boppart, {Stephen A.}",
note = "Funding Information: Funding for this project was provided by GlaxoSmithKline through the Center for Optical Molecular Imaging, located at the Beckman Institute for Advanced Science and Technology on the campus of the University of Illinois at Urbana-Champaign. The FLIMfit software tool was developed at Imperial College London and used under a GNU public “copyleft” license. We thank Dr. Haohua Tu for his advice and support in the laser source development. S.A.B. and H.T. are co-founders of LiveBx, LLC, which is commercializing optical source technology related to this research.The current affiliation for Zane Arp is the U.S. Food and Drug Administration, Silver Spring, MD, USA. Funding Information: GlaxoSmithKline Funding for this project was provided by GlaxoSmithKline through the Center for Optical Molecular Imaging, located at the Beckman Institute for Advanced Science and Technology on the campus of the University of Illinois at Urbana-Champaign. The FLIMfit software tool was developed at Imperial College London and used under a GNU public ?copyleft? license. We thank Dr. Haohua Tu for his advice and support in the laser source development. S.A.B. and H.T. are co-founders of LiveBx, LLC, which is commercializing optical source technology related to this research.The current affiliation for Zane Arp is the U.S. Food and Drug Administration, Silver Spring, MD, USA. Publisher Copyright: {\textcopyright} 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement",
year = "2019",
month = oct,
day = "1",
doi = "10.1364/BOE.10.005431",
language = "English (US)",
volume = "10",
pages = "5431--5444",
journal = "Biomedical Optics Express",
issn = "2156-7085",
publisher = "The Optical Society",
number = "10",
}