Simplified formation of thin-shell double emulsions for robust single entity analysis

Thomas Cowell, Andrew Dobria, Hee Sun Han

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Double emulsions have found new applications for biomedical research relying on their exceptional emulsion stability that enables drop sorting on commercial FACS. To improve the accessibility of double emulsions techniques, we demonstrate minimally complex methods for double emulsion generation that match existing methods while simplifying fabrication and surface treatment. We further probed the stability of these double emulsions and compared them with equivalent single emulsions. Double emulsions showed excellent stability during thermal cycling and displayed unique merging behavior that ensures emulsion contents do not mix, which is critical for single cell applications.

Original languageEnglish (US)
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1035-1036
Number of pages2
ISBN (Electronic)9781733419031
StatePublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: Oct 10 2021Oct 14 2021

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period10/10/2110/14/21

Keywords

  • Double Emulsions
  • Droplet Microfluidics
  • Single cell analysis
  • ddPCR

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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