TY - GEN
T1 - Simplified formation of thin-shell double emulsions for robust single entity analysis
AU - Cowell, Thomas
AU - Dobria, Andrew
AU - Han, Hee Sun
N1 - Funding Information:
This work was supported by the University of Illinois at Urbana-Champaign. Fabrication was carried out in part at the Materials Research Laboratory Central Research Facilities, University of Illinois.
Funding Information:
Using a single layer device and simplified surface treatments, it is possible to generate high-quality monodisperse double emulsions with minimal expertise. Additional control over the shell thickness was obtained using droplet shearing. These drops have idea properties for PCR in drops and FACS sorting. We compared the stability and merging dynamics of these double emulsions, verifying that they compare favorably to existing single emulsion techniques across multiple criteria. ACKNOWLEDGEMENTS This work was supported by the University of Illinois at Urbana-Champaign. Fabrication was carried out in part at the Materials Research Laboratory Central Research Facilities, University of Illinois. REFERENCES [1] K. K. Brower, “Double emulsion flow cytometry”, Lab Chip, 2020, 20, 2062–2074.
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Double emulsions have found new applications for biomedical research relying on their exceptional emulsion stability that enables drop sorting on commercial FACS. To improve the accessibility of double emulsions techniques, we demonstrate minimally complex methods for double emulsion generation that match existing methods while simplifying fabrication and surface treatment. We further probed the stability of these double emulsions and compared them with equivalent single emulsions. Double emulsions showed excellent stability during thermal cycling and displayed unique merging behavior that ensures emulsion contents do not mix, which is critical for single cell applications.
AB - Double emulsions have found new applications for biomedical research relying on their exceptional emulsion stability that enables drop sorting on commercial FACS. To improve the accessibility of double emulsions techniques, we demonstrate minimally complex methods for double emulsion generation that match existing methods while simplifying fabrication and surface treatment. We further probed the stability of these double emulsions and compared them with equivalent single emulsions. Double emulsions showed excellent stability during thermal cycling and displayed unique merging behavior that ensures emulsion contents do not mix, which is critical for single cell applications.
KW - ddPCR
KW - Double Emulsions
KW - Droplet Microfluidics
KW - Single cell analysis
UR - http://www.scopus.com/inward/record.url?scp=85136967062&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136967062&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136967062
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1035
EP - 1036
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -