Simple sheath flow reactor for post-column fluorescence derivatization in capillary electrophoresis

Kurt E. Oldenburg, Xiaoyan Xi, Jonathan V. Sweedler

Research output: Contribution to journalArticlepeer-review

Abstract

A system for post-column fluorescence derivatization in capillary electrophoresis is described. The post-column reactor uses a sheath flow detection cell where the reagents, o-phthaldialdehyde and β-mercaptoethanol, are added to the sheath buffer and mix by diffusion with the analytes effusing from the separation capillary. Reaction progress is monitored and optimized by imaging a large portion of the sheath flow cuvette using an extended UV source and a CCD camera. Significantly, this design provides the ability to switch between the analysis of pre- and post-column derivatized amino acids and peptides easily and without sacrificing system performance. The lack of turbulent flow in this system minimizes post-separation band broadening. The limit of detection for glycine is 9.4 x 10-8 M (110 amol) with a separation efficiency of 190,000 theoretical plates, without stacking. The performance of the system for a series of amino acids was evaluated using post-column and pre-capillary derivatization.

Original languageEnglish (US)
Pages (from-to)1581-1585
Number of pages5
JournalAnalyst
Volume122
Issue number12
DOIs
StatePublished - 1997

Keywords

  • Capillary electrophoresis
  • Fluorescence
  • O-phthaldialdehyde
  • Post-column derivatization
  • Sheath flow cell

ASJC Scopus subject areas

  • Analytical Chemistry

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