Silver staining of DNA in polyacrylamide gels

Brant J. Bassam, Gustavo Caetano-Anollés

Research output: Contribution to journalArticlepeer-review

Abstract

Nucleic acids can be detected at the picogram level using a quick and simple silver staining method (2). Using very thin polyesterbacked polyacrylamide gels, a further simplified protocol was compared to other widely used silver staining procedures. The improved protocol described here was the most sensitive, the fastest to perform, and had relatively few steps and reagents. This method also produced the least number of staining artifacts and offered images of high contrast.

Original languageEnglish (US)
Pages (from-to)181-188
Number of pages8
JournalApplied Biochemistry and Biotechnology
Volume42
Issue number2-3
DOIs
StatePublished - Aug 1 1993
Externally publishedYes

Keywords

  • DNA
  • polyacrylamide gels
  • silver staining

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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