Abstract
Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O 2-reduction activity while generating little reactive oxygen species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4312-4316 |
| Number of pages | 5 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 51 |
| Issue number | 18 |
| DOIs | |
| State | Published - Apr 27 2012 |
Keywords
- enzyme catalysis
- enzyme models
- oxygen reduction
- post-translational modifications
- protein design
ASJC Scopus subject areas
- General Chemistry
- Catalysis