@article{0bc7bcd380f64e8d9cd4f06a74282909,
title = "Shape-fitting collagen-PLA composite promotes osteogenic differentiation of porcine adipose stem cells",
abstract = "Craniomaxillofacial bone defects can occur as a result of congenital, post-oncologic, and high-energy impact conditions. The scale and irregularity of such defects motivate new biomaterials to promote regeneration of the damaged bone. We have recently described a mineralized collagen scaffold capable of instructing stem cell osteogenic differentiation and new bone infill in the absence of traditional osteogenic supplements. Herein, we report the integration of a millimeter-scale reinforcing poly (lactic acid) frame fabricated via 3D-printing into the mineralized collagen scaffold with micron-scale porosity to form a multi-scale mineralized collagen-PLA composite. We describe modifications to the PLA frame design to increase the compressive strength (Young's Modulus, ultimate stress and strain) of the composite. A critical challenge beyond increasing the compressive strength of the collagen scaffold is addressing challenges inherent with the irregularity of clinical defects. As a result, we examined the potential for modifying the frame architecture to render the composite with increased compressive strength in one axis or radial compressibility and shape-fitting capacity in an orthogonal axis. A library of mineralized collagen-PLA composites was mechanically characterized via compression testing and push-out test to describe mechanical performance and shape-fitting capacity. We also report in vitro comparison of the bioactivity of porcine adipose derived stem cells in the mineralized collagen-PLA composite versus the mineralized collagen scaffold via metabolic activity, gene expression, and functional matrix synthesis. The results suggest that incorporation of the PLA reinforcing frame does not negatively influence the osteoinductive nature of the mineralized collagen scaffold. Together, these findings suggest a strategy to address often competing bioactivity, mechanical strength, and shape-fitting design requirements for biomaterials for craniomaxillofacial bone regeneration.",
keywords = "Collagen, Conformal fitting, Osteogenesis, Poly lactic acid, Stem cell",
author = "Dewey, \{Marley J.\} and Johnson, \{Eileen M.\} and Weisgerber, \{Daniel W.\} and Wheeler, \{Matthew B.\} and Harley, \{Brendan A.C.\}",
note = "The authors would like to acknowledge the Carl R. Woese Institute for Genomic Biology for assistance with Western blot analysis, Kingsley Boateng for help with Nanozoomer training, and Derek Milner for assistance with histology. The authors would like to acknowledge the Illinois Makerlab and Vishal Sachdev for use of 3D printers and assistance printing PLA reinforcements. This research was carried out in part at the Imaging Technology Group within the Beckman Institute for Advanced Science and Technology at the University of Illinois at Urbana-Champaign; the authors would like to thank Leilei Yin for assistance with Micro-CT, and Scott Robinson and Cate Wallace for assistance with ESEM. The authors would also like to acknowledge the Roy J. Carver Biotechnology Center and assistance with RT-PCR from Tatsiana Akraiko and Mark Band. This work was supported by the Office of the Assistant Secretary of Defense for Health Affairs Broad Agency Announcement for Extramural Medical Research through the Award No. W81XWH-16-1-0566 . Opinions, interpretations, conclusions and recommendations are those of the authors and are not necessarily endorsed by the Department of Defense. Research reported in this publication was also supported by the National Institute of Dental and Craniofacial Research of the National Institutes of Health under Award Number R21 DE026582 . The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. We are grateful for the funding for this study provided by the NSF Graduate Research Fellowship DGE-1144245 (MJD). The authors would like to acknowledge the Carl R. Woese Institute for Genomic Biology for assistance with Western blot analysis, Kingsley Boateng for help with Nanozoomer training, and Derek Milner for assistance with histology. The authors would like to acknowledge the Illinois Makerlab and Vishal Sachdev for use of 3D printers and assistance printing PLA reinforcements. This research was carried out in part at the Imaging Technology Group within the Beckman Institute for Advanced Science and Technology at the University of Illinois at Urbana-Champaign; the authors would like to thank Leilei Yin for assistance with Micro-CT, and Scott Robinson and Cate Wallace for assistance with ESEM. The authors would also like to acknowledge the Roy J. Carver Biotechnology Center and assistance with RT-PCR from Tatsiana Akraiko and Mark Band. This work was supported by the Office of the Assistant Secretary of Defense for Health Affairs Broad Agency Announcement for Extramural Medical Research through the Award No. W81XWH-16-1-0566. Opinions, interpretations, conclusions and recommendations are those of the authors and are not necessarily endorsed by the Department of Defense. Research reported in this publication was also supported by the National Institute of Dental and Craniofacial Research of the National Institutes of Health under Award Number R21 DE026582. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. We are grateful for the funding for this study provided by the NSF Graduate Research Fellowship DGE-1144245 (MJD).",
year = "2019",
month = jul,
doi = "10.1016/j.jmbbm.2019.03.017",
language = "English (US)",
volume = "95",
pages = "21--33",
journal = "Journal of the Mechanical Behavior of Biomedical Materials",
issn = "1751-6161",
publisher = "Elsevier Ltd",
}