Sex-and tissue-specific expression of a cytochrome P450 2C2-luciferase transgene 1

Hong Li, Siqing Liu, Byron Kemper

Research output: Contribution to journalArticlepeer-review

Abstract

To study the transcriptional regulation of the rabbit cytochrome P450 2C2 gene (CYP2C2), transgenic mice were generated using a DNA fragment containing the CYP2C2 5′-flanking region fused to a luciferase reporter gene. In one of three transgenic lines, this transgene was expressed in the liver and at a lower level in the kidney, similar to the tissue-specific pattern of this gene in rabbits. Unlike expression in rabbits, the transgene was expressed in a male-specific pattern in liver and kidney, and in the brain of both sexes. Expression of the transgene in male mice was repressed after castration, while expression in females was stimulated after testosterone treatment and periodic injection of rat growth hormone (GH). The expression of this transgene was also regulated developmentally in the liver. These results suggest that this 3500 bp of CYP2C2 5′-flanking region contains the basal transcriptional regulation elements for tissue-specific and developmental expression. Although a position effect can not be ruled out, this transgene may also include the cis-regulatory element for sexual dimorphic expression.

Original languageEnglish (US)
Pages (from-to)77-83
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume120
Issue number1
DOIs
StatePublished - Jun 18 1996

Keywords

  • CYP2C2
  • Growth hormone
  • Male-specific
  • Phenobarbital
  • Testosterone
  • Transgenic mice

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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