Serum cortisol mediates the relationship between fecal Ruminococcus and brain N-acetylaspartate in the young pig

Austin T. Mudd, Kirsten Berding, Mei Wang, Sharon M. Donovan, Ryan N. Dilger

Research output: Contribution to journalArticle

Abstract

A dynamic relationship between the gut microbiota and brain is pivotal in neonatal development. Dysbiosis of the microbiome may result in altered neurodevelopment; however, it is unclear which specific members of microbiota are most influential and what factors might mediate the relationship between the gut and the brain. Twenty-four vaginally-derived male piglets were subjected to magnetic resonance spectroscopy at 30 d of age. Ascending colon contents, feces, and blood were collected and analyzed for volatile fatty acids, microbiota relative abundance by 16s rRNA, and serum metabolites, respectively. A mediation analysis was performed to assess the mediatory effect of serum biomarkers on the relationship between microbiota and neurometabolites. Results indicated fecal Ruminococcus and Butyricimonas predicted brain N-acetylaspartate (NAA). Analysis of serum biomarkers indicated Ruminococcus independently predicted serum serotonin and cortisol. A 3-step mediation indicated: i) Ruminococcus negatively predicted NAA, ii) Ruminococcus negatively predicted cortisol, and iii) a significant indirect effect (i.e., the effect of fecal Ruminococcus through cortisol on NAA) was observed and the direct effect became insignificant. Thus, serum cortisol fully mediated the relationship between fecal Ruminococcus and brain NAA. Using magnetic resonance spectroscopy, this study used a statistical mediation analysis and provides a novel perspective into the potential underlying mechanisms through which the microbiota may shape brain development. This is the first study to link Ruminococcus, cortisol, and NAA in vivo, and these findings are substantiated by previous literature indicating these factors may be influential in the etiology of neurodevelopmental disorders.

Original languageEnglish (US)
Pages (from-to)589-600
Number of pages12
JournalGut Microbes
Volume8
Issue number6
DOIs
StatePublished - Nov 2 2017

Fingerprint

Ruminococcus
Hydrocortisone
Microbiota
Swine
Brain
Serum
Magnetic Resonance Spectroscopy
Biomarkers
Dysbiosis
Ascending Colon
Volatile Fatty Acids
N-acetylaspartate
Feces
Serotonin

Keywords

  • N-acetylaspartate
  • Ruminococcus
  • autism
  • microbiome
  • neurodevelopment
  • pig

ASJC Scopus subject areas

  • Microbiology
  • Gastroenterology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Serum cortisol mediates the relationship between fecal Ruminococcus and brain N-acetylaspartate in the young pig. / Mudd, Austin T.; Berding, Kirsten; Wang, Mei; Donovan, Sharon M.; Dilger, Ryan N.

In: Gut Microbes, Vol. 8, No. 6, 02.11.2017, p. 589-600.

Research output: Contribution to journalArticle

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