SERS driven cross-platform based multiplex pathogen detection

Sandeep P. Ravindranath, Yuling Wang, Joseph Irudayaraj

Research output: Contribution to journalArticle

Abstract

We demonstrate a cross-platform approach to simultaneously detect three different pathogens using Raman and UV-vis absorption spectroscopy. Gold (Au), silver (Ag), and Ag-Au core-shell nanoparticles were functionalized with anti-Salmonella typhimurium aptamers, anti-Staphylococcus aureus and anti-Escherichia coli O157:H7 antibodies respectively and labeled with unique Raman reporter molecules. A microfiltration step was used to consolidate a highly selective and specific detection platform, with total detection time under 45 min for both species (E. coli O157:H7 vs. S. typhimurium) and strain (E. coli O157:H7 vs. E. coli K12) level sensing at a limit of a detection ranging between 102 and 103 CFU/ml. This simple yet robust multiplex detection platform has the potential to be developed into a rapid and portable pathogen sensor for ultrasensitive detection in liquid samples.

Original languageEnglish (US)
Pages (from-to)183-190
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume152
Issue number2
DOIs
StatePublished - Mar 1 2011
Externally publishedYes

Fingerprint

pathogens
Pathogens
Escherichia coli
platforms
Salmonella
Microfiltration
Ultraviolet spectroscopy
Absorption spectroscopy
Silver
Antibodies
Gold
salmonella
staphylococcus
Escherichia
Nanoparticles
antibodies
Molecules
Sensors
absorption spectroscopy
Liquids

Keywords

  • Aptamers
  • Cross-platform detection
  • Multiplex detection
  • Nanotechnology
  • Pathogens
  • SERS

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

SERS driven cross-platform based multiplex pathogen detection. / Ravindranath, Sandeep P.; Wang, Yuling; Irudayaraj, Joseph.

In: Sensors and Actuators, B: Chemical, Vol. 152, No. 2, 01.03.2011, p. 183-190.

Research output: Contribution to journalArticle

Ravindranath, Sandeep P. ; Wang, Yuling ; Irudayaraj, Joseph. / SERS driven cross-platform based multiplex pathogen detection. In: Sensors and Actuators, B: Chemical. 2011 ; Vol. 152, No. 2. pp. 183-190.
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