SERS driven cross-platform based multiplex pathogen detection

Sandeep P. Ravindranath; Yuling Wang; Joseph Irudayaraj

doi:10.1016/j.snb.2010.12.005

SERS driven cross-platform based multiplex pathogen detection

Sandeep P. Ravindranath, Yuling Wang, Joseph Irudayaraj

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrate a cross-platform approach to simultaneously detect three different pathogens using Raman and UV-vis absorption spectroscopy. Gold (Au), silver (Ag), and Ag-Au core-shell nanoparticles were functionalized with anti-Salmonella typhimurium aptamers, anti-Staphylococcus aureus and anti-Escherichia coli O157:H7 antibodies respectively and labeled with unique Raman reporter molecules. A microfiltration step was used to consolidate a highly selective and specific detection platform, with total detection time under 45 min for both species (E. coli O157:H7 vs. S. typhimurium) and strain (E. coli O157:H7 vs. E. coli K12) level sensing at a limit of a detection ranging between 10² and 10³ CFU/ml. This simple yet robust multiplex detection platform has the potential to be developed into a rapid and portable pathogen sensor for ultrasensitive detection in liquid samples.

Original language	English (US)
Pages (from-to)	183-190
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	152
Issue number	2
DOIs	https://doi.org/10.1016/j.snb.2010.12.005
State	Published - Mar 1 2011
Externally published	Yes

Keywords

Aptamers
Cross-platform detection
Multiplex detection
Nanotechnology
Pathogens
SERS

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

Online availability

10.1016/j.snb.2010.12.005

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title = "SERS driven cross-platform based multiplex pathogen detection",

abstract = "We demonstrate a cross-platform approach to simultaneously detect three different pathogens using Raman and UV-vis absorption spectroscopy. Gold (Au), silver (Ag), and Ag-Au core-shell nanoparticles were functionalized with anti-Salmonella typhimurium aptamers, anti-Staphylococcus aureus and anti-Escherichia coli O157:H7 antibodies respectively and labeled with unique Raman reporter molecules. A microfiltration step was used to consolidate a highly selective and specific detection platform, with total detection time under 45 min for both species (E. coli O157:H7 vs. S. typhimurium) and strain (E. coli O157:H7 vs. E. coli K12) level sensing at a limit of a detection ranging between 102 and 103 CFU/ml. This simple yet robust multiplex detection platform has the potential to be developed into a rapid and portable pathogen sensor for ultrasensitive detection in liquid samples.",

keywords = "Aptamers, Cross-platform detection, Multiplex detection, Nanotechnology, Pathogens, SERS",

author = "Ravindranath, {Sandeep P.} and Yuling Wang and Joseph Irudayaraj",

note = "Funding Information: This research was supported by the Center for Food Safety Engineering grant (USDA-Purdue), Purdue University. We would like to acknowledge Dr. Pavan Kumar for help with design and synthesis of silver and Ag–Au core–shell nanoparticles. We would like to thank Dr.Chitrita Deb-Roy for providing the bacterial strains. We would like to thank Dr.Srinand Sreevatsan for the gift of aptamers. ",

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AB - We demonstrate a cross-platform approach to simultaneously detect three different pathogens using Raman and UV-vis absorption spectroscopy. Gold (Au), silver (Ag), and Ag-Au core-shell nanoparticles were functionalized with anti-Salmonella typhimurium aptamers, anti-Staphylococcus aureus and anti-Escherichia coli O157:H7 antibodies respectively and labeled with unique Raman reporter molecules. A microfiltration step was used to consolidate a highly selective and specific detection platform, with total detection time under 45 min for both species (E. coli O157:H7 vs. S. typhimurium) and strain (E. coli O157:H7 vs. E. coli K12) level sensing at a limit of a detection ranging between 102 and 103 CFU/ml. This simple yet robust multiplex detection platform has the potential to be developed into a rapid and portable pathogen sensor for ultrasensitive detection in liquid samples.

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SERS driven cross-platform based multiplex pathogen detection

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