Serotonin receptor gene expression in the rat suprachiasmatic nuclei

Alfred L Roca, David R. Weaver, Steven M. Reppert

Research output: Contribution to journalArticle

Abstract

Serotonin (5HT) is thought to reset the biological clock in the suprachiasmatic nuclei (SCN) in vitro through a postsynaptic 5HT-1a receptor. Thus we examined 5HT receptor gene expression in the SCN by in situ hybridization. On film autoradiograms, 5HT-1c receptor mRNA showed intense SCN hybridization, while 5HT-1b receptor mRNA displayed a weaker signal. Emulsion autoradiograms additionally revealed expression of 5HT-1a and 5HT-2 receptor mRNAs by a few scattered SCN cells. 5HT-3 receptor mRNA was not detected in the SCN, although the transcript was detected elsewhere in the brain. 5HT-1d and -1e receptor mRNAs were not detected in the SCN or elsewhere in brain within the sections examined. The results do not support a major role for postsynaptic 5HT-1a receptors in resetting SCN rhythms.

Original languageEnglish (US)
Pages (from-to)159-165
Number of pages7
JournalBrain Research
Volume608
Issue number1
DOIs
StatePublished - Apr 9 1993
Externally publishedYes

Keywords

  • Nucleic acid hybridization
  • Serotonin receptor
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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