Serotonin induces parathyroid hormone-related protein in goat mammary gland

W. J. Zang, H. Li, Z. F. Zhang, R. QuZhen, Y. Z. CuoMu, D. K. Zhang, J. Luo, J. J. Loor, H. L. Zheng

Research output: Contribution to journalArticle

Abstract

During lactation, large amounts of calcium are exported from the mammary gland into milk to ensure skeletal growth of the offspring. Recent studies revealed that serotonin (5-HT) is essential to stimulate skeletal calcium resorption for milk synthesis. Our objective was to explore the correlation between circulating 5-HT and serum calcium and parathyroid hormone-related protein (PTHrP) concentrations around parturition in dairy goats. We also investigated the effect of 5-HT on PTHrP expression in cultured primary goat mammary epithelial cells (GMEC). Blood samples of multiparous Guanzhong dairy goats were collected on day −5 to 3 postpartum for analysis of serum concentrations of calcium, 5-HT, and PTHrP. Results revealed that from day −3 to 0 postpartum serum calcium and 5-HT concentrations decreased progressively, but serum PTHrP concentration only had a sharp drop in the postpartum period sampled. Correlation analysis of circulating 5-HT and serum calcium and PTHrP concentrations on day 1 and 2 postpartum revealed that low serum 5-HT concentration was positively correlated with serum total calcium or PTHrP concentration. By knocking down trypto-phan hydroxylase-1 (TPH1) or adding 5-hydrox-ytryptophan (5-HTP) to decrease or increase the levels of 5-HT in GMEC, we observed that 5-HTP increased PTHrP expression in a dose-dependent manner and siTPH1 decreased PTHrP protein expression. Furthermore, 5-HT increased mRNA abundance of calcium-sensing receptor (CaSR) in a dose-dependent manner and decreased the expression of plasma membrane Ca2+ ATPase-1 (PMCA1). Taken together, 5-HT seems to induce PTHrP expression in goat mammary cells during and after parturition. These findings suggest that increasing 5-HT biosynthesis could be a potential therapeutic target for prevention of hypocalcemia in dairy goats.

Original languageEnglish (US)
Pages (from-to)1010-1016
Number of pages7
JournalJournal of animal science
Volume96
Issue number3
DOIs
StatePublished - Apr 3 2018

Fingerprint

Parathyroid Hormone-Related Protein
parathyroid hormone
Human Mammary Glands
serotonin
Goats
mammary glands
Serotonin
goats
blood serum
calcium
proteins
Calcium
Postpartum Period
dairy goats
protein synthesis
Serum
breasts
Breast
epithelial cells
Epithelial Cells

Keywords

  • 5-HT
  • Calcium
  • Goat
  • PTHrP

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Zang, W. J., Li, H., Zhang, Z. F., QuZhen, R., CuoMu, Y. Z., Zhang, D. K., ... Zheng, H. L. (2018). Serotonin induces parathyroid hormone-related protein in goat mammary gland. Journal of animal science, 96(3), 1010-1016. https://doi.org/10.1093/jas/skx023

Serotonin induces parathyroid hormone-related protein in goat mammary gland. / Zang, W. J.; Li, H.; Zhang, Z. F.; QuZhen, R.; CuoMu, Y. Z.; Zhang, D. K.; Luo, J.; Loor, J. J.; Zheng, H. L.

In: Journal of animal science, Vol. 96, No. 3, 03.04.2018, p. 1010-1016.

Research output: Contribution to journalArticle

Zang, WJ, Li, H, Zhang, ZF, QuZhen, R, CuoMu, YZ, Zhang, DK, Luo, J, Loor, JJ & Zheng, HL 2018, 'Serotonin induces parathyroid hormone-related protein in goat mammary gland', Journal of animal science, vol. 96, no. 3, pp. 1010-1016. https://doi.org/10.1093/jas/skx023
Zang WJ, Li H, Zhang ZF, QuZhen R, CuoMu YZ, Zhang DK et al. Serotonin induces parathyroid hormone-related protein in goat mammary gland. Journal of animal science. 2018 Apr 3;96(3):1010-1016. https://doi.org/10.1093/jas/skx023
Zang, W. J. ; Li, H. ; Zhang, Z. F. ; QuZhen, R. ; CuoMu, Y. Z. ; Zhang, D. K. ; Luo, J. ; Loor, J. J. ; Zheng, H. L. / Serotonin induces parathyroid hormone-related protein in goat mammary gland. In: Journal of animal science. 2018 ; Vol. 96, No. 3. pp. 1010-1016.
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abstract = "During lactation, large amounts of calcium are exported from the mammary gland into milk to ensure skeletal growth of the offspring. Recent studies revealed that serotonin (5-HT) is essential to stimulate skeletal calcium resorption for milk synthesis. Our objective was to explore the correlation between circulating 5-HT and serum calcium and parathyroid hormone-related protein (PTHrP) concentrations around parturition in dairy goats. We also investigated the effect of 5-HT on PTHrP expression in cultured primary goat mammary epithelial cells (GMEC). Blood samples of multiparous Guanzhong dairy goats were collected on day −5 to 3 postpartum for analysis of serum concentrations of calcium, 5-HT, and PTHrP. Results revealed that from day −3 to 0 postpartum serum calcium and 5-HT concentrations decreased progressively, but serum PTHrP concentration only had a sharp drop in the postpartum period sampled. Correlation analysis of circulating 5-HT and serum calcium and PTHrP concentrations on day 1 and 2 postpartum revealed that low serum 5-HT concentration was positively correlated with serum total calcium or PTHrP concentration. By knocking down trypto-phan hydroxylase-1 (TPH1) or adding 5-hydrox-ytryptophan (5-HTP) to decrease or increase the levels of 5-HT in GMEC, we observed that 5-HTP increased PTHrP expression in a dose-dependent manner and siTPH1 decreased PTHrP protein expression. Furthermore, 5-HT increased mRNA abundance of calcium-sensing receptor (CaSR) in a dose-dependent manner and decreased the expression of plasma membrane Ca2+ ATPase-1 (PMCA1). Taken together, 5-HT seems to induce PTHrP expression in goat mammary cells during and after parturition. These findings suggest that increasing 5-HT biosynthesis could be a potential therapeutic target for prevention of hypocalcemia in dairy goats.",
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AU - CuoMu, Y. Z.

AU - Zhang, D. K.

AU - Luo, J.

AU - Loor, J. J.

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