@article{60f46215df164b84b347c09d3dc9eb3a,
title = "Sequential sequestrations increase the incorporation and retention of multiple growth factors in mineralized collagen scaffolds",
abstract = "Trauma induced injuries of the mouth, jaw, face, and related structures present unique clinical challenges due to their large size and complex geometry. Growth factor signaling coordinates the behavior of multiple cell types following an injury, and effective coordination of growth factor availability within a biomaterial can be critical for accelerating bone healing. Mineralized collagen scaffolds are a class of degradable biomaterial whose biophysical and compositional parameters can be adjusted to facilitate cell invasion and tissue remodeling. Here we describe the use of modified simulated body fluid treatments to enable sequential sequestration of bone morphogenic protein 2 and vascular endothelial growth factor into mineralized collagen scaffolds for bone repair. We report the capability of these scaffolds to sequester 60-90% of growth factor from solution without additional crosslinking treatments and show high levels of retention for individual (>94%) and multiple growth factors (>88%) that can be layered into the material via sequential sequestration steps. Sequentially sequestering growth factors allows prolonged release of growth factors in vitro (>94%) and suggests the potential to improve healing of large-scale bone injury models in vivo. Future work will utilize this sequestration method to induce cellular activities critical to bone healing such as vessel formation and cell migration.",
author = "Tiffany, {Aleczandria S.} and Dewey, {Marley J.} and Harley, {Brendan A.C.}",
note = "Funding Information: This work was supported by the Office of the Assistant Secretary of Defense for Health Affairs Broad Agency Announcement for Extramural Medical Research through the Award No. W81XWH-16-1-0566. Opinions, interpretations, conclusions and recommendations are those of the authors and are not necessarily endorsed by the Department of Defense. Research reported in this publication was also supported by the National Institute of Dental and Craniofacial Research of the National Institutes of Health under Award Number R21 DE026582. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. We are grateful for the funding for this study provided by the NSF Graduate Research Fellowship DGE-1144245 (AST) and DGE-1144245 (MJD). The authors would like to acknowledge the University of Illinois Roy J. Carver Biotechnology Center for assistance with real-time PCR. This research was carried out in part at the Imaging Technology Group within the Beckman Institute for Advanced Science and Technology at the University of Illinois at Urbana-Champaign. The authors would like to thank Scott Robinson and Cate Wallace for assistance with critical point drying and scanning electron microscopy. Additional support was provided by the Chemical and Biomolecular Engineering Department and the Carl R. Woese Institute for Genomic Biology (BACH) at the University of Illinois at Urbana-Champaign.",
year = "2020",
month = jun,
day = "30",
doi = "10.1039/d0ra03872e",
language = "English (US)",
volume = "10",
pages = "26982--26996",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "45",
}