Sequence and structure of a phenylalanine ammonia-lyase gene from glycine max

Ronald L. Frank, Lila O. Vodkin

Research output: Contribution to journalArticle

Abstract

The gene encoding a key enzyme in anthocyanin biosynthesis, phenylalanine ammonia-lyase (PAL), was cloned from soybean (Glycine max). The purpose was to obtain a molecular probe to study the organization of this gene family in soybean and to examine novel regulatory mechanisms present in the anthocyanin biosynthetic pathway of this system. A soybean genomic library was constructed in the bacteriophage vector lambda Charon 35. A PAL cDNA clone from Phaseolus vulgaris was used in screening the library, and two PAL genes were isolated. One gene was sequenced entirely and analyzed by sequence homology to the PAL2 gene of Phaseolus vulgaris. Genomic analysis indicates that PAL sequences of Glycine max exist as a small gene family consisting of only two to three members. The representative PAL gene sequenced (PAL1) has a coding region of 2142 basepairs divided among two exons. The single intron is 1519 basepairs and splits the 131st codon.

Original languageEnglish (US)
Pages (from-to)335-346
Number of pages12
JournalMitochondrial DNA
Volume1
Issue number5
DOIs
StatePublished - Jan 1 1991

Keywords

  • Anthocyanin biosynthesis
  • Flavonoids
  • General phenylpropanoid metabolism
  • PAL gene
  • Soybean

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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