Separation Of Lanthanide-Binding Cells

Marshall D. Graham, Paul R. Selvin

Research output: Contribution to journalArticle

Abstract

A new method for magnetic separation of cells is described. Based on cellular concentration of complexed paramagnetic cations from a carrier solution, the method achieves highly repeatable separations, at lower magnetization fields, than existing HGMS cell-separation methods. Feasibility has been shown for erbium and dysprosium, with whole blood as the sample. Single-pass magnetic separation efficiencies significantly greater than those attainable with paramagnetic erythrocytic hemoglobin are readily available, e.g., 90% versus 37%, at 0.3 Tesla and 0.75ml/min flow rate. The method requires no cellular incubations, is not technically demanding, and does not visibly affect cellular integrity; it promises to provide a flexible and general technique for cell separation.

Original languageEnglish (US)
Pages (from-to)1523-1525
Number of pages3
JournalIEEE Transactions on Magnetics
Volume18
Issue number6
DOIs
StatePublished - Nov 1982
Externally publishedYes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering

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