Separating beads and cells in multi-channel microfluidic devices using dielectrophoresis and laminar flow

Larry J. Millet, Kidong Park, Nicholas N. Watkins, K. Jimmy Hsia, Rashid Bashir

Research output: Contribution to journalArticle

Abstract

Microfluidic devices have advanced cell studies by providing a dynamic fluidic environment on the scale of the cell for studying, manipulating, sorting and counting cells. However, manipulating the cell within the fluidic domain remains a challenge and requires complicated fabrication protocols for forming valves and electrodes, or demands specialty equipment like optical tweezers. Here, we demonstrate that conventional printed circuit boards (PCB) can be used for the non-contact manipulation of cells by employing dielectrophoresis (DEP) for bead and cell manipulation in laminar flow fields for bioactuation, and for cell and bead separation in multichannel microfluidic devices. First, we present the protocol for assembling the DEP electrodes and microfluidic devices, and preparing the cells for DEP. Then, we characterize the DEP operation with polystyrene beads. Lastly, we show representative results of bead and cell separation in a multichannel microfluidic device. In summary, DEP is an effective method for manipulating particles (beads or cells) within microfluidic devices.

Original languageEnglish (US)
Article numbere2545
JournalJournal of Visualized Experiments
Issue number48
DOIs
StatePublished - Feb 2011

Fingerprint

Lab-On-A-Chip Devices
Electrophoresis
Laminar flow
Microfluidics
Fluidics
Cell Separation
Optical tweezers
Electron tubes
Electrodes
Polystyrenes
Sorting
Printed circuit boards
Optical Tweezers
Flow fields
Fabrication

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Separating beads and cells in multi-channel microfluidic devices using dielectrophoresis and laminar flow. / Millet, Larry J.; Park, Kidong; Watkins, Nicholas N.; Jimmy Hsia, K.; Bashir, Rashid.

In: Journal of Visualized Experiments, No. 48, e2545, 02.2011.

Research output: Contribution to journalArticle

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