Sensitive on-chip detection of a protein biomarker in human serum and plasma over an extended dynamic range using silicon photonic microring resonators and sub-micron beads

Matthew S. Luchansky, Adam L. Washburn, Melinda S. McClellan, Ryan C. Bailey

Research output: Contribution to journalArticle

Abstract

We demonstrate a three-step assay on a silicon photonic microring resonator-based detection platform that enables the quantitation of the cardiac biomarker C-reactive protein (CRP) over a dynamic range spanning six orders of magnitude. Using antibody-modified microrings, we sequentially monitor primary CRP binding, secondary recognition of bound CRP by a biotinylated antibody, and tertiary signal amplification using streptavidin-functionalized beads. This detection methodology is applied to CRP quantitation in human serum and plasma samples.

Original languageEnglish (US)
Pages (from-to)2042-2044
Number of pages3
JournalLab on a chip
Volume11
Issue number12
DOIs
StatePublished - Jun 21 2011

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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