Semiautomated Analysis of Alkaline Phosphatase Isoenzymes in Serum of Normal Cynomolgus Monkeys (Macaca fascicularis)

Charles E. Wiedmeyer, Daniel G. Morton, Patrick K. Cusick, Thomas Wright, Philip F. Solter, Walter E. Hoffmann

Research output: Contribution to journalArticlepeer-review

Abstract

Alkaline phosphatase (ALP) isoenzyme analysis, using a combination of wheat germ lectin (WGL) precipitation, levamisole inhibition and an automated p-nitrophenylphosphate assay was validated for use with serum from monkeys (Macaca fascicularis) and used to determine the activities of liver ALP (LALP), bone ALP (BALP) and intestinal ALP (IALP). Based on serial dilution studies and within-run and between-run coefficients of variation, each assay had excellent linearity and acceptable precision. In addition, liver and intestinal mucosa extracts for tissue specific alkaline phosphatases were used to confirm assay validations. Gender-specific differences for total ALP, LALP and BALP activities were present in sera from normal monkeys between 2 and 4 years of age. Males had 1.3-fold higher total ALP and LALP activities, and 1.5-fold higher BALP activity compared with females. The majority of ALP activity in normal monkey serum was LALP isoenzyme activity, which ranged from 56.7% to 94.7% of total activity. Serum BALP activity ranged from 5.3%. to 42.8%. There was negligible IALP activity in all serum samples.

Original languageEnglish (US)
Pages (from-to)2-7
Number of pages6
JournalVeterinary Clinical Pathology
Volume28
Issue number1
DOIs
StatePublished - Jan 1 1999

Keywords

  • Alkaline phosphatase
  • Bone
  • Intestine
  • Levamisole
  • Liver
  • Wheat germ lectin

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint

Dive into the research topics of 'Semiautomated Analysis of Alkaline Phosphatase Isoenzymes in Serum of Normal Cynomolgus Monkeys (Macaca fascicularis)'. Together they form a unique fingerprint.

Cite this