TY - JOUR
T1 - SEM and TEM study of the hierarchical structure of C57BL/6J and C3H/HeJ mice trabecular bone
AU - Rubin, Matthew A.
AU - Rubin, Janet
AU - Jasiuk, Iwona
N1 - Funding Information:
I.J. and J.R. acknowledge the support of the Emory/Georgia Tech Seed Grant Program 1999-2000 and I.J. acknowledges the support of the National Science Foundation (Grant CMS-0085137). The SEM and TEM work was conducted in the Integrated Microscopy and Microanalytical Facility at Emory University (Dr. Robert Apkarian, director). The authors would like to thank Dr. R. Apkarian and J. Taylor, the technologist, for their assistance in preparation and viewing of samples, and Mr. Andrew Yoo for his help in dissecting samples.
PY - 2004/7
Y1 - 2004/7
N2 - Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to study the hierarchical structure of trabecular bone from C57BL/6J (low bone mass) and C3H/HeJ mice (high bone mass). Bone was harvested from two different anatomical locations: femoral metaphysis and L5 vertebra. This investigation focused on three structural scales: the mesostructural (porous network of trabecular struts), the microstructural (collagen fibril arrangements in trabecular packets), and the nanostructural (collagen fibril and apatite crystals) levels. At the mesostructural level, no distinct differences were found in the trabecular structure of femoral metaphysis but thinner trabecular struts were observed in L5 vertebra for C57BL/6J mice strain. At the microstructural level, the collagen fibrils forming the rotated, twisted, and orthogonal plywood arrangements were distinguished as well as atypical arrangements. At the nanostructural level, the shape and size of apatite crystals, and their arrangement with respect to collagen fibrils were studied. In spite of very different bone mass densities, both mice strains had similar structures at the nanostructural and microstructural levels.
AB - Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to study the hierarchical structure of trabecular bone from C57BL/6J (low bone mass) and C3H/HeJ mice (high bone mass). Bone was harvested from two different anatomical locations: femoral metaphysis and L5 vertebra. This investigation focused on three structural scales: the mesostructural (porous network of trabecular struts), the microstructural (collagen fibril arrangements in trabecular packets), and the nanostructural (collagen fibril and apatite crystals) levels. At the mesostructural level, no distinct differences were found in the trabecular structure of femoral metaphysis but thinner trabecular struts were observed in L5 vertebra for C57BL/6J mice strain. At the microstructural level, the collagen fibrils forming the rotated, twisted, and orthogonal plywood arrangements were distinguished as well as atypical arrangements. At the nanostructural level, the shape and size of apatite crystals, and their arrangement with respect to collagen fibrils were studied. In spite of very different bone mass densities, both mice strains had similar structures at the nanostructural and microstructural levels.
KW - Bone hierarchical structure
KW - C3H/HeJ and C57/6J mice
KW - Scanning electron microscopy
KW - Trabecular bone
KW - Transmission electron microscopy
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U2 - 10.1016/j.bone.2004.02.008
DO - 10.1016/j.bone.2004.02.008
M3 - Article
C2 - 15207736
AN - SCOPUS:2942718862
SN - 8756-3282
VL - 35
SP - 11
EP - 20
JO - Bone
JF - Bone
IS - 1
ER -