Abstract
Surface plasmon resonance (SPR) is a rapid and label-free detection technique for the detection of pathogenic microorganisms. A preliminary study to investigate the capability of an alkanethiol mixed self assembled monolayer (SAM) based SPR biosensor to detect pathogens was demonstrated. A protocol for the detection of pathogens suspended in phosphate buffered saline with Tween 20 (PBST) (pH 7.4) was developed. Antisera against Escherichia coli O157:H7 suspended in sodium acetate (NaOAc) (pH 5.5) was immobilized on an activated sensor chip. Escherichia coli O157:H7 broth was diluted in PBST and passed over the immobilized antibodies to run a direct assay. For sandwich assays anti-Escherichia coli O157:H7 sera suspended in PBST was passed over the bound bacteria. Sandwich assay produced an enhanced signal compared to direct assay. Results indicate that a SAM based SPR biosensor has the potential for rapid detection of pathogens by monitoring the biomolecular interactions on the sensor surface.
| Original language | English (US) |
|---|---|
| Pages | 7805-7812 |
| Number of pages | 8 |
| State | Published - 2004 |
| Externally published | Yes |
| Event | ASAE Annual International Meeting 2004 - Ottawa, ON, Canada Duration: Aug 1 2004 → Aug 4 2004 |
Other
| Other | ASAE Annual International Meeting 2004 |
|---|---|
| Country/Territory | Canada |
| City | Ottawa, ON |
| Period | 8/1/04 → 8/4/04 |
Keywords
- Pathogen Detection
- Self Assembled Monolayers
- Surface Plasmon Resonance
ASJC Scopus subject areas
- General Engineering