Selective membrane redistribution and depletion of Gαq-protein by Pasteurella multocida toxin

Nathan C. Clemons, Shuhong Luo, Mengfei Ho, Brenda A. Wilson

Research output: Contribution to journalArticle

Abstract

Pasteurella multocida toxin (PMT), the major virulence factor responsible for zoonotic atrophic rhinitis, is a protein deamidase that activates the alpha subunit of heterotrimeric G proteins. Initial activation of G alpha-q-coupled phospholipase C-beta-1 signaling by PMT is followed by uncoupling of G alpha-q-dependent signaling, causing downregulation of downstream calcium and mitogenic signaling pathways. Here, we show that PMT decreases endogenous and exogenously expressed G alpha-q protein content in host cell plasma membranes and in detergent resistant membrane (DRM) fractions. This membrane depletion of G alpha-q protein was dependent upon the catalytic activity of PMT. Results indicate that PMT-modified G alpha-q redistributes within the host cell membrane from the DRM fraction into the soluble membrane and cytosolic fractions. In contrast, PMT had no affect on G alpha-s or G beta protein levels, which are not substrate targets of PMT. PMT also had no affect on G alpha-11 levels, even though G alpha-11 can serve as a substrate for deamidation by PMT, suggesting that membrane depletion of PMT-modified G-alpha-q has specificity.

Original languageEnglish (US)
Article number233
JournalToxins
Volume8
Issue number8
DOIs
StatePublished - Aug 1 2016

Keywords

  • Deamidation
  • Dermonecrosis
  • G-protein downregulation
  • Mitogen
  • Pasteurella multocida

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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