Selection of bacterial virulence genes that are specifically induced in host tissues

Michael J. Mahan; James M. Slauch; John J. Mekalanos

doi:10.1126/science.8430319

Selection of bacterial virulence genes that are specifically induced in host tissues

Michael J. Mahan, James M. Slauch, John J. Mekalanos

Research output: Contribution to journal › Article › peer-review

Abstract

A genetic system was devised that positively selects for bacterial genes that are specifically induced when bacteria infect their host. With the pathogen Salmonella typhimurium, the genes identified by this selection show a marked induction in bacteria recovered from mouse spleen. Mutations in all ivi (in vivo-induced) genes that were tested conferred a defect in virulence. This genetic system was designed to be of general use in a wide variety of bacterial-host systems and has several applications in both vaccine and antimicrobial drug development.

Original language	English (US)
Pages (from-to)	686-688
Number of pages	3
Journal	Science
Volume	259
Issue number	5095
DOIs	https://doi.org/10.1126/science.8430319
State	Published - Jan 29 1993
Externally published	Yes

ASJC Scopus subject areas

General

Online availability

10.1126/science.8430319

Library availability

Discover UIUC Full Text

Cite this

@article{de4e891d50fe462386cc3ec623da4adb,

title = "Selection of bacterial virulence genes that are specifically induced in host tissues",

abstract = "A genetic system was devised that positively selects for bacterial genes that are specifically induced when bacteria infect their host. With the pathogen Salmonella typhimurium, the genes identified by this selection show a marked induction in bacteria recovered from mouse spleen. Mutations in all ivi (in vivo-induced) genes that were tested conferred a defect in virulence. This genetic system was designed to be of general use in a wide variety of bacterial-host systems and has several applications in both vaccine and antimicrobial drug development.",

author = "Mahan, {Michael J.} and Slauch, {James M.} and Mekalanos, {John J.}",

year = "1993",

month = jan,

day = "29",

doi = "10.1126/science.8430319",

language = "English (US)",

volume = "259",

pages = "686--688",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5095",

}

TY - JOUR

T1 - Selection of bacterial virulence genes that are specifically induced in host tissues

AU - Mahan, Michael J.

AU - Slauch, James M.

AU - Mekalanos, John J.

PY - 1993/1/29

Y1 - 1993/1/29

N2 - A genetic system was devised that positively selects for bacterial genes that are specifically induced when bacteria infect their host. With the pathogen Salmonella typhimurium, the genes identified by this selection show a marked induction in bacteria recovered from mouse spleen. Mutations in all ivi (in vivo-induced) genes that were tested conferred a defect in virulence. This genetic system was designed to be of general use in a wide variety of bacterial-host systems and has several applications in both vaccine and antimicrobial drug development.

AB - A genetic system was devised that positively selects for bacterial genes that are specifically induced when bacteria infect their host. With the pathogen Salmonella typhimurium, the genes identified by this selection show a marked induction in bacteria recovered from mouse spleen. Mutations in all ivi (in vivo-induced) genes that were tested conferred a defect in virulence. This genetic system was designed to be of general use in a wide variety of bacterial-host systems and has several applications in both vaccine and antimicrobial drug development.

UR - http://www.scopus.com/inward/record.url?scp=0027497926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027497926&partnerID=8YFLogxK

U2 - 10.1126/science.8430319

DO - 10.1126/science.8430319

M3 - Article

C2 - 8430319

AN - SCOPUS:0027497926

SN - 0036-8075

VL - 259

SP - 686

EP - 688

JO - Science

JF - Science

IS - 5095

ER -

Selection of bacterial virulence genes that are specifically induced in host tissues

Abstract

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this