Selection and characterization of a carrot cell line tolerant to glyphosate

Emerson D Nafziger, Jack M. Widholm, Hans C. Steinrücken, John L. Killmer

Research output: Contribution to journalArticle

Abstract

Cultured carrot (Daucs carota L.) cells were adapted to growing in 25 millimolar glyphosate by transfer into progressively higher concentrations ofthe herbicide. Tolerance was increased 52-fold, and the adaptation was stable in the absence of glyphosate. The uptake of glyphosate was similar for adapted and nonadapted cells. Activity of the enzyme 5- enolpyruvylshikimic acid-3-phosphate synthase was 12-fold higher in the adapted line compared to nonadapted cells, while activities of shikimate dehydrogenase and anthranilate synthase were similr in the two cell types. The adapted cells had higher levels of free amino acids-especially threonine, methionine, tyrosine, phenylalanine, tryptophan, histidine, and arginine than did nonadapted cells. Glyphosate treatment caused decreases of 50 to 65% in the levels of serine, glycine, methionine, tyrosine, phenybaanine, and tryptophan in nonadapted cells, but caused little change in free amino acid levels in adapted cells. The adaptation reported here supports the growing body of evidence linking tolerance to glyphosate with increased levels of the enzyme 5- enolpyruvylshikimic acid-3-phosphate synthase. The elevated levels of aromatic amino acids, which may confer resistance in adapted cells, suggest that control of the shikimate pathway may be altered in these cells.

Original languageEnglish (US)
Pages (from-to)571-574
Number of pages4
JournalPlant physiology
Volume76
Issue number3
DOIs
StatePublished - Jan 1 1984

Fingerprint

glyphosate
Daucus carota
carrots
cell lines
Cell Line
cells
3-Phosphoshikimate 1-Carboxyvinyltransferase
Tryptophan
Methionine
tryptophan
free amino acids
Tyrosine
tyrosine
methionine
Anthranilate Synthase
anthranilate synthase
phosphates
shikimate pathway
Amino Acids
Aromatic Amino Acids

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Nafziger, E. D., Widholm, J. M., Steinrücken, H. C., & Killmer, J. L. (1984). Selection and characterization of a carrot cell line tolerant to glyphosate. Plant physiology, 76(3), 571-574. https://doi.org/10.1104/pp.76.3.571

Selection and characterization of a carrot cell line tolerant to glyphosate. / Nafziger, Emerson D; Widholm, Jack M.; Steinrücken, Hans C.; Killmer, John L.

In: Plant physiology, Vol. 76, No. 3, 01.01.1984, p. 571-574.

Research output: Contribution to journalArticle

Nafziger, ED, Widholm, JM, Steinrücken, HC & Killmer, JL 1984, 'Selection and characterization of a carrot cell line tolerant to glyphosate', Plant physiology, vol. 76, no. 3, pp. 571-574. https://doi.org/10.1104/pp.76.3.571
Nafziger ED, Widholm JM, Steinrücken HC, Killmer JL. Selection and characterization of a carrot cell line tolerant to glyphosate. Plant physiology. 1984 Jan 1;76(3):571-574. https://doi.org/10.1104/pp.76.3.571
Nafziger, Emerson D ; Widholm, Jack M. ; Steinrücken, Hans C. ; Killmer, John L. / Selection and characterization of a carrot cell line tolerant to glyphosate. In: Plant physiology. 1984 ; Vol. 76, No. 3. pp. 571-574.
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