Segmental pairs of dermal secretory cells release proteins into the hemolymph at the larval-pupal molt

James Nardi, Lou Ann Miller, Hugh M. Robertson, Peter M. Yau

Research output: Contribution to journalArticlepeer-review

Abstract

At each molt of Manduca, the large dermal secretory cells expel the protein contents of their vacuoles into the hemocoel. The constellation of proteins expelled at the last larval-pupal molt, however, differs qualitatively from those proteins released at earlier larval-larval molts. Secretory cells at the two stages not only have different lectin staining properties but also have different proteins that separate on two-dimensional gels. Numerous physiological changes accompany the termination of the last larval instar, including increased chitin synthesis, diminished oxygen delivery, and reduced humoral immunity. Secretion of trehalase that is essential for chitin synthesis and the release of hypoxia up-regulated protein to ameliorate oxygen deprivation help ensure normal transition from larva to pupa. Proteins released by dermal secretory cells at this last molt could supplement the diminished immune defenses mediated by fat body and hemocytes at the end of larval life. Additional immune defenses provided by dermal secretory cells could help ensure a safe transition during a period of increased vulnerability for the newly molted pupa with its soft, thin cuticle and reduced mobility.

Original languageEnglish (US)
Pages (from-to)107-111
Number of pages5
JournalDevelopmental Biology
Volume483
DOIs
StatePublished - Mar 2022

Keywords

  • Dermal gland
  • Hemolymph
  • Immune proteins
  • Larval-pupal molt
  • Protein secretion
  • Verson's organs

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Developmental Biology

Fingerprint

Dive into the research topics of 'Segmental pairs of dermal secretory cells release proteins into the hemolymph at the larval-pupal molt'. Together they form a unique fingerprint.

Cite this