Seed-based expression strategies

Lucia Orellana-Escobedo, Schuyler S. Korban, Sergio Rosales-Mendoza

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Among the current molecular pharming approaches, seed-based expression offers particular advantages as heterologous proteins can be produced at high levels in a highly stable biomass that can also serve as an oral vaccine after either minimal or no processing. Therefore, seeds can be readily used as platforms for the production of low-cost vaccines that are environmentally friendly and easy to administer. In this chapter, various strategies that address expression of immunogens in seeds are presented. Seed-based approaches that have been undertaken to develop candidate vaccines against cholera, malaria, hepatitis B, and diarrhea are featured as examples of the promising potential of these approaches. Moreover, insights into the immunogenic properties of seed-based candidate vaccines are explored. In addition, efforts that prevent undesirable gene flow and release of transgene(s) into the environment are also explored.

Original languageEnglish (US)
Title of host publicationGenetically Engineered Plants as a Source of Vaccines Against Wide Spread Diseases
Subtitle of host publicationAn Integrated View
PublisherSpringer New York
Pages79-93
Number of pages15
ISBN (Electronic)9781493908509
ISBN (Print)1493908499, 9781493908493
DOIs
StatePublished - Mar 1 2014

Keywords

  • Biopharmaceuticals
  • Immune responses
  • Molecular pharming
  • Seed-based candidate vaccine
  • Transgenic seed

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Orellana-Escobedo, L., Korban, S. S., & Rosales-Mendoza, S. (2014). Seed-based expression strategies. In Genetically Engineered Plants as a Source of Vaccines Against Wide Spread Diseases: An Integrated View (pp. 79-93). Springer New York. https://doi.org/10.1007/978-1-4939-0850-9_5