Secretion and cell surface expression of IgG1 are impaired in human B lymphoblasts that lack HLA-A, -B, and -C antigens

W. J. Burlingham, Stephanie S Ceman, R. DeMars

Research output: Contribution to journalComment/debate

Abstract

B-lymphoblastoid cell line (LCL) 721.221 lacks HLA-A, -B, and -C class I antigens and transcripts as a result of γ-ray-induced mutations. LCL 721, from which mutant .221 was derived, produces membrane and secreted forms of IgG1(κ). In contrast, IgG expression in .221 had these characteristics: (i) γ1 heavy chains were diminished by 98% but were detectable with chain-specific antibodies in cell lysates; (ii) κ light chains were present at normal levels in cell lysates and free κ chains were secreted; (iii) cell-surface-associated IgG and secreted IgG were absent. Mutants that had partially reduced amounts of class I antigens continued to secrete IgG; however, both the absolute amount of IgG secreted and the relative amount of κ vs. intact IgG secreted were abnormal in such partially class I-deficient cells. The failure to export IgG and the deficiency of HLA-A, -B, and -C were not merely coincidental in mutant .221, since production of IgG was restored by transferring a functional HLA-A, -B or -C gene into .221. Cell surface antigen expression of cloned HLA-A, -B, and -C transgenes introduced into .221 was comparable to that of the same genes in their normal chromosomal locations. These observations reveal a relation between production of HLA class I gene products and production of IgG.

Original languageEnglish (US)
Pages (from-to)8005-8009
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number20
DOIs
StatePublished - Jan 1 1989

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HLA-A Antigens
HLA-B Antigens
Immunoglobulin G
Antigens
Histocompatibility Antigens Class I
IgG Deficiency
MHC Class I Genes
Cell Line
Surface Antigens
Transgenes
Genes
Light
Mutation
Membranes
Antibodies

ASJC Scopus subject areas

  • General

Cite this

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title = "Secretion and cell surface expression of IgG1 are impaired in human B lymphoblasts that lack HLA-A, -B, and -C antigens",
abstract = "B-lymphoblastoid cell line (LCL) 721.221 lacks HLA-A, -B, and -C class I antigens and transcripts as a result of γ-ray-induced mutations. LCL 721, from which mutant .221 was derived, produces membrane and secreted forms of IgG1(κ). In contrast, IgG expression in .221 had these characteristics: (i) γ1 heavy chains were diminished by 98{\%} but were detectable with chain-specific antibodies in cell lysates; (ii) κ light chains were present at normal levels in cell lysates and free κ chains were secreted; (iii) cell-surface-associated IgG and secreted IgG were absent. Mutants that had partially reduced amounts of class I antigens continued to secrete IgG; however, both the absolute amount of IgG secreted and the relative amount of κ vs. intact IgG secreted were abnormal in such partially class I-deficient cells. The failure to export IgG and the deficiency of HLA-A, -B, and -C were not merely coincidental in mutant .221, since production of IgG was restored by transferring a functional HLA-A, -B or -C gene into .221. Cell surface antigen expression of cloned HLA-A, -B, and -C transgenes introduced into .221 was comparable to that of the same genes in their normal chromosomal locations. These observations reveal a relation between production of HLA class I gene products and production of IgG.",
author = "Burlingham, {W. J.} and Ceman, {Stephanie S} and R. DeMars",
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T1 - Secretion and cell surface expression of IgG1 are impaired in human B lymphoblasts that lack HLA-A, -B, and -C antigens

AU - Burlingham, W. J.

AU - Ceman, Stephanie S

AU - DeMars, R.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - B-lymphoblastoid cell line (LCL) 721.221 lacks HLA-A, -B, and -C class I antigens and transcripts as a result of γ-ray-induced mutations. LCL 721, from which mutant .221 was derived, produces membrane and secreted forms of IgG1(κ). In contrast, IgG expression in .221 had these characteristics: (i) γ1 heavy chains were diminished by 98% but were detectable with chain-specific antibodies in cell lysates; (ii) κ light chains were present at normal levels in cell lysates and free κ chains were secreted; (iii) cell-surface-associated IgG and secreted IgG were absent. Mutants that had partially reduced amounts of class I antigens continued to secrete IgG; however, both the absolute amount of IgG secreted and the relative amount of κ vs. intact IgG secreted were abnormal in such partially class I-deficient cells. The failure to export IgG and the deficiency of HLA-A, -B, and -C were not merely coincidental in mutant .221, since production of IgG was restored by transferring a functional HLA-A, -B or -C gene into .221. Cell surface antigen expression of cloned HLA-A, -B, and -C transgenes introduced into .221 was comparable to that of the same genes in their normal chromosomal locations. These observations reveal a relation between production of HLA class I gene products and production of IgG.

AB - B-lymphoblastoid cell line (LCL) 721.221 lacks HLA-A, -B, and -C class I antigens and transcripts as a result of γ-ray-induced mutations. LCL 721, from which mutant .221 was derived, produces membrane and secreted forms of IgG1(κ). In contrast, IgG expression in .221 had these characteristics: (i) γ1 heavy chains were diminished by 98% but were detectable with chain-specific antibodies in cell lysates; (ii) κ light chains were present at normal levels in cell lysates and free κ chains were secreted; (iii) cell-surface-associated IgG and secreted IgG were absent. Mutants that had partially reduced amounts of class I antigens continued to secrete IgG; however, both the absolute amount of IgG secreted and the relative amount of κ vs. intact IgG secreted were abnormal in such partially class I-deficient cells. The failure to export IgG and the deficiency of HLA-A, -B, and -C were not merely coincidental in mutant .221, since production of IgG was restored by transferring a functional HLA-A, -B or -C gene into .221. Cell surface antigen expression of cloned HLA-A, -B, and -C transgenes introduced into .221 was comparable to that of the same genes in their normal chromosomal locations. These observations reveal a relation between production of HLA class I gene products and production of IgG.

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