Secondary-site attachment of coliphage lambda near the thr operon

Jeffrey F. Gardner; Oliver H. Smith; Walter W. Fredricks; Michael A. McKinney

doi:10.1016/0022-2836(74)90528-2

Secondary-site attachment of coliphage lambda near the thr operon

Jeffrey F. Gardner, Oliver H. Smith, Walter W. Fredricks, Michael A. McKinney

Research output: Contribution to journal › Article › peer-review

Abstract

Phage λ has been integrated at a secondary attachment site near the thr oporon of Escherichia coli. The integration caused a pleiotropic effect since both thrA and thrB enzyme activities were lost. Lysogenic, thr⁺ revertants regained thrA and thrB activities although the enzymes were synthetized constitutively at low levels. Four classes of prophage deletion strains were isolated with deletions extending into trpR and the thr operon. Genetic and biochemical analyses indicated a gene order: trpR .. λ .. thrA .. B .. C. Defective transducing phage carrying thr A, B,C were also isolated.

Original language	English (US)
Pages (from-to)	613-631
Number of pages	19
Journal	Journal of Molecular Biology
Volume	90
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(74)90528-2
State	Published - Dec 25 1974
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(74)90528-2

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@article{a64f5c59c013444db17f1cd08ed0419a,

title = "Secondary-site attachment of coliphage lambda near the thr operon",

abstract = "Phage λ has been integrated at a secondary attachment site near the thr oporon of Escherichia coli. The integration caused a pleiotropic effect since both thrA and thrB enzyme activities were lost. Lysogenic, thr+ revertants regained thrA and thrB activities although the enzymes were synthetized constitutively at low levels. Four classes of prophage deletion strains were isolated with deletions extending into trpR and the thr operon. Genetic and biochemical analyses indicated a gene order: trpR .. λ .. thrA .. B .. C. Defective transducing phage carrying thr A, B,C were also isolated.",

author = "Gardner, {Jeffrey F.} and Smith, {Oliver H.} and Fredricks, {Walter W.} and McKinney, {Michael A.}",

note = "Funding Information: We thank Drs Cohen, Gottesman, Salivar and Weisberg for generously providing phage and bacterial strains, and especially Drs Schrenk and Weisberg for communicating their recent results before publication. This research was supported by grants from the National Institute of General Medical Sciences (GM18494) and from the Research Corporation. The senior author (J.F.G.) was the recipient of a National Defense Education Act Title IV fellowship and a Marquette University fellowship. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1974",

month = dec,

day = "25",

doi = "10.1016/0022-2836(74)90528-2",

language = "English (US)",

volume = "90",

pages = "613--631",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press Inc.",

number = "4",

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TY - JOUR

T1 - Secondary-site attachment of coliphage lambda near the thr operon

AU - Gardner, Jeffrey F.

AU - Smith, Oliver H.

AU - Fredricks, Walter W.

AU - McKinney, Michael A.

N1 - Funding Information: We thank Drs Cohen, Gottesman, Salivar and Weisberg for generously providing phage and bacterial strains, and especially Drs Schrenk and Weisberg for communicating their recent results before publication. This research was supported by grants from the National Institute of General Medical Sciences (GM18494) and from the Research Corporation. The senior author (J.F.G.) was the recipient of a National Defense Education Act Title IV fellowship and a Marquette University fellowship. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1974/12/25

Y1 - 1974/12/25

N2 - Phage λ has been integrated at a secondary attachment site near the thr oporon of Escherichia coli. The integration caused a pleiotropic effect since both thrA and thrB enzyme activities were lost. Lysogenic, thr+ revertants regained thrA and thrB activities although the enzymes were synthetized constitutively at low levels. Four classes of prophage deletion strains were isolated with deletions extending into trpR and the thr operon. Genetic and biochemical analyses indicated a gene order: trpR .. λ .. thrA .. B .. C. Defective transducing phage carrying thr A, B,C were also isolated.

AB - Phage λ has been integrated at a secondary attachment site near the thr oporon of Escherichia coli. The integration caused a pleiotropic effect since both thrA and thrB enzyme activities were lost. Lysogenic, thr+ revertants regained thrA and thrB activities although the enzymes were synthetized constitutively at low levels. Four classes of prophage deletion strains were isolated with deletions extending into trpR and the thr operon. Genetic and biochemical analyses indicated a gene order: trpR .. λ .. thrA .. B .. C. Defective transducing phage carrying thr A, B,C were also isolated.

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U2 - 10.1016/0022-2836(74)90528-2

DO - 10.1016/0022-2836(74)90528-2

M3 - Article

C2 - 4615174

AN - SCOPUS:0016334653

VL - 90

SP - 613

EP - 631

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 4

ER -

Secondary-site attachment of coliphage lambda near the thr operon

Abstract

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