TY - JOUR
T1 - Secondary-site attachment of coliphage lambda near the thr operon
AU - Gardner, Jeffrey F.
AU - Smith, Oliver H.
AU - Fredricks, Walter W.
AU - McKinney, Michael A.
N1 - Funding Information:
We thank Drs Cohen, Gottesman, Salivar and Weisberg for generously providing phage and bacterial strains, and especially Drs Schrenk and Weisberg for communicating their recent results before publication. This research was supported by grants from the National Institute of General Medical Sciences (GM18494) and from the Research Corporation. The senior author (J.F.G.) was the recipient of a National Defense Education Act Title IV fellowship and a Marquette University fellowship.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1974/12/25
Y1 - 1974/12/25
N2 - Phage λ has been integrated at a secondary attachment site near the thr oporon of Escherichia coli. The integration caused a pleiotropic effect since both thrA and thrB enzyme activities were lost. Lysogenic, thr+ revertants regained thrA and thrB activities although the enzymes were synthetized constitutively at low levels. Four classes of prophage deletion strains were isolated with deletions extending into trpR and the thr operon. Genetic and biochemical analyses indicated a gene order: trpR .. λ .. thrA .. B .. C. Defective transducing phage carrying thr A, B,C were also isolated.
AB - Phage λ has been integrated at a secondary attachment site near the thr oporon of Escherichia coli. The integration caused a pleiotropic effect since both thrA and thrB enzyme activities were lost. Lysogenic, thr+ revertants regained thrA and thrB activities although the enzymes were synthetized constitutively at low levels. Four classes of prophage deletion strains were isolated with deletions extending into trpR and the thr operon. Genetic and biochemical analyses indicated a gene order: trpR .. λ .. thrA .. B .. C. Defective transducing phage carrying thr A, B,C were also isolated.
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U2 - 10.1016/0022-2836(74)90528-2
DO - 10.1016/0022-2836(74)90528-2
M3 - Article
C2 - 4615174
AN - SCOPUS:0016334653
VL - 90
SP - 613
EP - 631
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 4
ER -