Secondary-site attachment of coliphage lambda near the thr operon

Jeffrey F. Gardner, Oliver H. Smith, Walter W. Fredricks, Michael A. McKinney

Research output: Contribution to journalArticlepeer-review


Phage λ has been integrated at a secondary attachment site near the thr oporon of Escherichia coli. The integration caused a pleiotropic effect since both thrA and thrB enzyme activities were lost. Lysogenic, thr+ revertants regained thrA and thrB activities although the enzymes were synthetized constitutively at low levels. Four classes of prophage deletion strains were isolated with deletions extending into trpR and the thr operon. Genetic and biochemical analyses indicated a gene order: trpR .. λ .. thrA .. B .. C. Defective transducing phage carrying thr A, B,C were also isolated.

Original languageEnglish (US)
Pages (from-to)613-631
Number of pages19
JournalJournal of Molecular Biology
Issue number4
StatePublished - Dec 25 1974
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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