Seasonal Patterns in Microbial Community Composition in Denitrifying Bioreactors Treating Subsurface Agricultural Drainage

Matthew D. Porter, J. Malia Andrus, Nicholas A. Bartolerio, Luis F Rodriguez, Yuanhui Zhang, Julie Zilles, Angela D Kent

Research output: Contribution to journalArticle

Abstract

Denitrifying bioreactors, consisting of water flow control structures and a woodchip-filled trench, are a promising approach for removing nitrate from agricultural subsurface or tile drainage systems. To better understand the seasonal dynamics and the ecological drivers of the microbial communities responsible for denitrification in these bioreactors, we employed microbial community “fingerprinting” techniques in a time-series examination of three denitrifying bioreactors over 2 years, looking at bacteria, fungi, and the denitrifier functional group responsible for the final step of complete denitrification. Our analysis revealed that microbial community composition responds to depth and seasonal variation in moisture content and inundation of the bioreactor media, as well as temperature. Using a geostatistical analysis approach, we observed recurring temporal patterns in bacterial and denitrifying bacterial community composition in these bioreactors, consistent with annual cycling. The fungal communities were more stable, having longer temporal autocorrelations, and did not show significant annual cycling. These results suggest a recurring seasonal cycle in the denitrifying bioreactor microbial community, likely due to seasonal variation in moisture content.

Original languageEnglish (US)
Pages (from-to)710-723
Number of pages14
JournalMicrobial Ecology
Volume70
Issue number3
DOIs
StatePublished - Oct 1 2015

Keywords

  • Denitrification
  • Geostatistics
  • Nitrate removal
  • Seasonal variation
  • Subsurface drainage

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science

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