Search for an endotherm in chloroplast lamellar membranes associated with chilling-inhibition of photosynthesis

P. S. Low, Donald Richard Ort, W. A. Cramer, J. Whitmarsh, B. Martin

Research output: Contribution to journalArticle

Abstract

The phase transition of chloroplast lamellar membrane lipids has been proposed to be the underlying cause of chilling-induced inhibition of photosynthesis in sensitive plants. Differential scanning calorimetry has been used to search for any endotherms arising from lipid state changes in chloroplast lamellar membranes of the chilling-sensitive plants cantaloupe, kidney bean, domestic tomato, and soybean. For comparison, calorimetric scans of chloroplast lamellar membranes from the chilling-insensitive plants spinach, pea, and wild tomato were made. A large reversible endotherm, extending from below 10 ° to nearly 40 °C, was observed in chloroplast membranes from tomatoes of both chilling-sensitive (Lycopersicon esculentum Mill. cv. Floramerica) and chillinginsensitive (L. hirsutum LA 1361) species. A much smaller endotherm, approximately 5 to 10% of the area of that seen in the two tomato species, and extending over a similar temperature range, was detected in chloroplasts from chilling-insensitive spinach and peas, and also was generally observed in chloroplasts from chilling-sensitive cantaloupe, kidney bean, and soybean. The enthalpy of these smaller endotherms indicates that, if the endotherm arose entirely from a lipid transition, then it corresponded to the melting of less than about 10% of the total membrane polar lipid. On the basis of these data it is concluded that there is no correlation between chilling sensitivity of photosynthesis and the presence or absence of a phase transition of bulk membrane lipids of the chloroplast lamellar membrane at temperatures above 5 °C.

Original languageEnglish (US)
Pages (from-to)336-344
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume231
Issue number2
DOIs
StatePublished - Jun 1984

Fingerprint

Chilling
Photosynthesis
Chloroplasts
Lycopersicon esculentum
Membranes
Membrane Lipids
Cucumis melo
Phaseolus
Spinacia oleracea
Phase Transition
Peas
Lipids
Soybeans
Phase transitions
Temperature
Differential Scanning Calorimetry
Freezing
Differential scanning calorimetry
Enthalpy
Melting

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Search for an endotherm in chloroplast lamellar membranes associated with chilling-inhibition of photosynthesis. / Low, P. S.; Ort, Donald Richard; Cramer, W. A.; Whitmarsh, J.; Martin, B.

In: Archives of Biochemistry and Biophysics, Vol. 231, No. 2, 06.1984, p. 336-344.

Research output: Contribution to journalArticle

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