Screening apples for OPD20/600 using sequence-specific primers

H. Yang, S. S. Korban

Research output: Contribution to journalArticlepeer-review

Abstract

Apple scab, caused by Venturia inaequalis (Cke.) Wint., is the most serious disease of apple trees in many areas of the world. Resistance to V. inaequalis, derived from the small-fruited species Malus floribunda 821, is determined by a major dominant gene, Vf. Using random decamer primers, we identified a RAPD marker, OPD20/600, which is linked to the Vf gene. OPD20/600 was then cloned and sequenced. Sequence-specific primers based on the marker were used to further screen M. floribunda 821, 7 scab- susceptible apple cultivars, 10 scab-resistant apple cultivars, and 28 scab- resistant Coop selections. The sequence-specific primers allowed identification of polymorphisms of OPD20/600 based on the presence or absence of a single band. The advantages of sequence-specific primers over decamer primers for developing genetic markers are discussed.

Original languageEnglish (US)
Pages (from-to)263-266
Number of pages4
JournalTheoretical and Applied Genetics
Volume92
Issue number2
DOIs
StatePublished - Feb 1996

Keywords

  • Apple
  • Apple scab resistance
  • Malus
  • RAPD marker
  • Venturia inaequalis

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Fingerprint

Dive into the research topics of 'Screening apples for OPD20/600 using sequence-specific primers'. Together they form a unique fingerprint.

Cite this