Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner

Alberto Brandariz-Núñez, Fuxing Zeng, Quan Ngoc Lam, J. I.N. Hong

Research output: Contribution to journalArticlepeer-review


RNA-binding protein Sbp1 facilitates the decapping pathway in mRNA metabolism and inhibits global mRNA translation by an unclear mechanism. Here we report molecular interactions responsible for Sbp1-mediated translation inhibition of mRNA encoding the polyadenosine-binding protein (Pab1), an essential translation factor that stimulates mRNA translation and inhibits mRNA decapping in eukaryotic cells. We demonstrate that the two distal RRMs of Sbp1 bind to the poly(A) sequence in the 5??UTR of the Pab1 mRNA specifically and cooperatively while the central RGG domain of the protein interacts directly with Pab1. Furthermore, methylation of arginines in the RGG domain abolishes the protein–protein interaction and the inhibitory effect of Sbp1 on translation initiation of Pab1 mRNA. Based on these results, the underlying mechanism for Sbp1-specific translational regulation is proposed. The functional differences of Sbp1 and RGG repeats alone on transcript-specific translation were observed, and a comparison of the results suggests the importance of remodeling the 5??UTR by RNA-binding proteins in mRNA translation.

Original languageEnglish (US)
Pages (from-to)43-55
Number of pages13
Issue number1
StatePublished - Jan 2018


  • 5??UTR
  • Polyadenosine-binding protein Pab1
  • RNA-binding protein
  • Sbp1
  • Translational regulation

ASJC Scopus subject areas

  • Molecular Biology


Dive into the research topics of 'Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner'. Together they form a unique fingerprint.

Cite this