Saturated absorption competition microscopy

Guangyuan Zhao, Mohammad M. Kabir, Kimani C. Toussaint, Cuifang Kuang, Cheng Zheng, Zhongzhi Yu, Xu Liu

Research output: Contribution to journalLetter

Abstract

Owing to the advantage of being non-invasive in observing living samples, far-field optical microscopy is widely used in the life sciences, but the existence of the diffraction barrier leads to the poor imaging of samples with spatial features smaller than approximately half the wavelength of the probes. This limit has been overcome by a number of pointwise scanning optical imaging techniques, such as stimulated emission depletion microscopy (STED) and saturated excitation microscopy (SAX). Here, we introduce the concept of saturated absorption competition (SAC) microscopy as a simple means of providing sub-diffraction spatial resolution in fluorescence imaging. Our approach can be physically implemented in a confocal microscope by dividing the input laser source into a time-modulated primary excitation beam and a doughnut-shaped saturation beam and subsequently employing a homodyne detection scheme to select the modulated fluorescence signal. Herein, we provide both a physico-chemical model of SAC and experimentally demonstrate a transverse spatial resolution of 1.5- to 2-fold that of confocal.

Original languageEnglish (US)
Pages (from-to)633-636
Number of pages4
JournalOptica
Volume4
Issue number6
DOIs
StatePublished - Jun 20 2017

Fingerprint

Microscopic examination
microscopy
Imaging techniques
Diffraction
Fluorescence
Homodyne detection
Stimulated emission
spatial resolution
fluorescence
life sciences
Optical microscopy
Light sources
Microscopes
stimulated emission
diffraction
imaging techniques
excitation
Scanning
far fields
Wavelength

Keywords

  • (110.0180) Microscopy
  • (180.2520) Fluorescence microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Zhao, G., Kabir, M. M., Toussaint, K. C., Kuang, C., Zheng, C., Yu, Z., & Liu, X. (2017). Saturated absorption competition microscopy. Optica, 4(6), 633-636. https://doi.org/10.1364/OPTICA.4.000633

Saturated absorption competition microscopy. / Zhao, Guangyuan; Kabir, Mohammad M.; Toussaint, Kimani C.; Kuang, Cuifang; Zheng, Cheng; Yu, Zhongzhi; Liu, Xu.

In: Optica, Vol. 4, No. 6, 20.06.2017, p. 633-636.

Research output: Contribution to journalLetter

Zhao, G, Kabir, MM, Toussaint, KC, Kuang, C, Zheng, C, Yu, Z & Liu, X 2017, 'Saturated absorption competition microscopy', Optica, vol. 4, no. 6, pp. 633-636. https://doi.org/10.1364/OPTICA.4.000633
Zhao G, Kabir MM, Toussaint KC, Kuang C, Zheng C, Yu Z et al. Saturated absorption competition microscopy. Optica. 2017 Jun 20;4(6):633-636. https://doi.org/10.1364/OPTICA.4.000633
Zhao, Guangyuan ; Kabir, Mohammad M. ; Toussaint, Kimani C. ; Kuang, Cuifang ; Zheng, Cheng ; Yu, Zhongzhi ; Liu, Xu. / Saturated absorption competition microscopy. In: Optica. 2017 ; Vol. 4, No. 6. pp. 633-636.
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AU - Liu, Xu

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