TY - JOUR
T1 - Satellite-tagged transcribing sequences in Bubalus bubalis genome undergo programmed modulation in meiocytes
T2 - Possible implications for transcriptional inactivation
AU - Chattopadhyay, Munmun
AU - Gangadharan, Supriya
AU - Kapur, Vipra
AU - Azfer, Mohammad Asim
AU - Prakash, Braham
AU - Ali, Sher
PY - 2001/9/1
Y1 - 2001/9/1
N2 - We cloned and sequenced a 1378 bp BamHI satellite DNA fraction from the water buffalo Bubalus bubalis and have studied its expression in different tissues. The GC-rich sequences of the resultant contig pDS5 crosshy-bridize only with bovid DNA and are not conserved evolutionarily. Typing of buffalo genomic DNA using pDS5 with several restriction enzymes revealed multilocus monomorphic bands. Similar typing of cattle, buffalo, goat, sheep, and gaur genomic DNA revealed variations in copy number and allele length giving rise to species-specific band patterns. Expression study of pDS5 in bubaline samples by RNA slot-blot, Northern blot, and RT-PCR showed various levels of signal in all the somatic tissues and germline cells except heart. A Gen-Bank database search revealed homology of pDS5 sequences in the 5′ region from nt 1-1261 with collagen gene. An AluI typing analysis of DNA from bubaline semen samples showed consistent loss of two bands. The presence of corresponding bands in somatic tissues suggests a sequence modulation within the pDS5 array in meiocytes during spermatogenesis, which is restored in the somatic cells after fertilization. Modulation of the satellite-tagged transcribing sequence in the meiocytes may be a mechanism of its inactivation.
AB - We cloned and sequenced a 1378 bp BamHI satellite DNA fraction from the water buffalo Bubalus bubalis and have studied its expression in different tissues. The GC-rich sequences of the resultant contig pDS5 crosshy-bridize only with bovid DNA and are not conserved evolutionarily. Typing of buffalo genomic DNA using pDS5 with several restriction enzymes revealed multilocus monomorphic bands. Similar typing of cattle, buffalo, goat, sheep, and gaur genomic DNA revealed variations in copy number and allele length giving rise to species-specific band patterns. Expression study of pDS5 in bubaline samples by RNA slot-blot, Northern blot, and RT-PCR showed various levels of signal in all the somatic tissues and germline cells except heart. A Gen-Bank database search revealed homology of pDS5 sequences in the 5′ region from nt 1-1261 with collagen gene. An AluI typing analysis of DNA from bubaline semen samples showed consistent loss of two bands. The presence of corresponding bands in somatic tissues suggests a sequence modulation within the pDS5 array in meiocytes during spermatogenesis, which is restored in the somatic cells after fertilization. Modulation of the satellite-tagged transcribing sequence in the meiocytes may be a mechanism of its inactivation.
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U2 - 10.1089/104454901317095007
DO - 10.1089/104454901317095007
M3 - Article
C2 - 11747610
AN - SCOPUS:0034756992
SN - 1044-5498
VL - 20
SP - 587
EP - 593
JO - DNA and Cell Biology
JF - DNA and Cell Biology
IS - 9
ER -