Abstract

Cells are extraordinarily complex, containing thousands of different analytes with concentrations spanning at least nine orders of magnitude. Analyzing single cells instead of tissue homogenates provides unique insights into cell-to-cell heterogeneity and aids in distinguishing normal cells from pathological ones. The high sensitivity and low sample consumption of capillary and on-chip electrophoresis, when integrated with fluorescence, electrochemical, and mass spectrometric detection methods, offer an ideal toolset for examining single cells and even subcellular organelles; however, the isolation and loading of such small samples into these devices is challenging. Recent advances have addressed this issue by interfacing a variety of enhanced mechanical, microfluidic, and optical sampling techniques to capillary and on-chip electrophoresis instruments for single-cell analyses.

Original languageEnglish (US)
Pages (from-to)2922-2929
Number of pages8
JournalAnalyst
Volume137
Issue number13
DOIs
StatePublished - Jul 7 2012

Fingerprint

Electrophoresis
electrokinesis
Sampling
detection method
Microfluidics
fluorescence
Fluorescence
Tissue
Single-Cell Analysis
Organelles
sampling technique
Equipment and Supplies
tissue
consumption

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Sampling techniques for single-cell electrophoresis. / Cecala, Christine; Sweedler, Jonathan V.

In: Analyst, Vol. 137, No. 13, 07.07.2012, p. 2922-2929.

Research output: Contribution to journalReview article

Cecala, Christine ; Sweedler, Jonathan V. / Sampling techniques for single-cell electrophoresis. In: Analyst. 2012 ; Vol. 137, No. 13. pp. 2922-2929.
@article{762e048b89034beb8f9a29576e270254,
title = "Sampling techniques for single-cell electrophoresis",
abstract = "Cells are extraordinarily complex, containing thousands of different analytes with concentrations spanning at least nine orders of magnitude. Analyzing single cells instead of tissue homogenates provides unique insights into cell-to-cell heterogeneity and aids in distinguishing normal cells from pathological ones. The high sensitivity and low sample consumption of capillary and on-chip electrophoresis, when integrated with fluorescence, electrochemical, and mass spectrometric detection methods, offer an ideal toolset for examining single cells and even subcellular organelles; however, the isolation and loading of such small samples into these devices is challenging. Recent advances have addressed this issue by interfacing a variety of enhanced mechanical, microfluidic, and optical sampling techniques to capillary and on-chip electrophoresis instruments for single-cell analyses.",
author = "Christine Cecala and Sweedler, {Jonathan V}",
year = "2012",
month = "7",
day = "7",
doi = "10.1039/c2an16211c",
language = "English (US)",
volume = "137",
pages = "2922--2929",
journal = "Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "13",

}

TY - JOUR

T1 - Sampling techniques for single-cell electrophoresis

AU - Cecala, Christine

AU - Sweedler, Jonathan V

PY - 2012/7/7

Y1 - 2012/7/7

N2 - Cells are extraordinarily complex, containing thousands of different analytes with concentrations spanning at least nine orders of magnitude. Analyzing single cells instead of tissue homogenates provides unique insights into cell-to-cell heterogeneity and aids in distinguishing normal cells from pathological ones. The high sensitivity and low sample consumption of capillary and on-chip electrophoresis, when integrated with fluorescence, electrochemical, and mass spectrometric detection methods, offer an ideal toolset for examining single cells and even subcellular organelles; however, the isolation and loading of such small samples into these devices is challenging. Recent advances have addressed this issue by interfacing a variety of enhanced mechanical, microfluidic, and optical sampling techniques to capillary and on-chip electrophoresis instruments for single-cell analyses.

AB - Cells are extraordinarily complex, containing thousands of different analytes with concentrations spanning at least nine orders of magnitude. Analyzing single cells instead of tissue homogenates provides unique insights into cell-to-cell heterogeneity and aids in distinguishing normal cells from pathological ones. The high sensitivity and low sample consumption of capillary and on-chip electrophoresis, when integrated with fluorescence, electrochemical, and mass spectrometric detection methods, offer an ideal toolset for examining single cells and even subcellular organelles; however, the isolation and loading of such small samples into these devices is challenging. Recent advances have addressed this issue by interfacing a variety of enhanced mechanical, microfluidic, and optical sampling techniques to capillary and on-chip electrophoresis instruments for single-cell analyses.

UR - http://www.scopus.com/inward/record.url?scp=84862177657&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862177657&partnerID=8YFLogxK

U2 - 10.1039/c2an16211c

DO - 10.1039/c2an16211c

M3 - Review article

C2 - 22288071

AN - SCOPUS:84862177657

VL - 137

SP - 2922

EP - 2929

JO - Analyst

JF - Analyst

SN - 0003-2654

IS - 13

ER -