Saliva-based diagnostics using 16S rRNA microarrays and microfluidics

E. Michelle Starke, James C. Smoot, Jer Horng Wu, Wen Tso Liu, Darrell Chandler, David A. Stahl

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The development of a diagnostic system based on DNA microarrays for rapid identification and enumeration of microbial species in the oral cavity is described. This system uses gel-based microarrays with immobilized probes designed within a phylogenetic framework that provides for comprehensive microbial monitoring. Understanding the community structure in the oral cavity is a necessary foundation onwhich to understand the breadth and depth of different microbial communities in the oral cavity and their role in acute and systemic disease. Our ultimate goal is to develop a diagnostic device to identify individuals at high risk for oral disease, and thereby reduce its prevalence and therefore the economic burden associated with treatment. This article discusses recent improvements of our system in reducing diffusional constraints in order to provide more rapid and accurate measurements of the microbial composition of saliva.

Original languageEnglish (US)
Title of host publicationOral-based Diagnostics
PublisherBlackwell Publishing Inc.
Pages345-361
Number of pages17
ISBN (Print)157331661X, 9781573316613
DOIs
StatePublished - Mar 2007
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1098
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Microarrays
  • Microbial ecology
  • Microfluidics
  • Saliva

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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