Saccharum × Miscanthus intergeneric hybrids (miscanes) exhibit greater chilling tolerance of C4 photosynthesis and postchilling recovery than sugarcane (Saccharum spp. hybrids)

Suraj Kar, Na Zhang, Taiken Nakashima, Antonio Villanueva-Morales, J. Ryan Stewart, Erik J. Sacks, Yoshifumi Terajima, Toshihiko Yamada

Research output: Contribution to journalArticle

Abstract

Although commercial sugarcane (Saccharum spp. hybrid) produces large biomass yields, its lack of cold tolerance limits its cultivation to the tropics and subtropics. In contrast, sugarcane's close relative, Miscanthus, tolerates low temperatures. We studied 18 miscane genotypes, derived from hybridizations between two genotypes of sugarcane and two genotypes of Miscanthus (one each of M. sinensis and M. sacchariflorus). In an initial greenhouse experiment on long-duration chilling stress (12–13°C day/7–9°C night), photosynthetic rates of the Miscanthus parents were significantly higher than the sugarcane parents after 7 days of chilling and were more than double by 14 days. The Miscanthus also retained more of their prechilling (22–25°C day/13–15°C night) photosynthetic rates (68%–72% 7 days, 64%–66% 14 days) than the sugarcanes (27% 7 days, 19%–20% 14 days). Seven of 18 miscanes exhibited higher photosynthetic rates than their sugarcane parents after 7 days of chilling, whereas after 14 days only four miscane genotypes had significantly higher photosynthetic rates than their sugarcane parents, but notably two of these did not differ from their highly tolerant Miscanthus parents. In a subsequent growth chamber experiment to evaluate short-duration chilling stress and postchilling recovery, three miscanes representing the range of responses observed in the greenhouse experiment were compared with their parents. After 4 days of chilling (12/7°C day/night), the miscanes retained between 45% and 60% of their prechilling photosynthetic rate, with the best entry not significantly different from its Miscanthus parent (66%), and all three miscanes performed significantly better than the sugarcane parents (32%–33% for sugarcanes). After 7 days of postchilling recovery (26/18°C day/night), the Miscanthus parents and two of the miscanes fully recovered their prechilling photosynthetic rates but the sugarcane parents only recovered 69%–73% of their prechilling rates. Thus, genes from Miscanthus can be used to improve chilling tolerance of sugarcane via introgression.

Original languageEnglish (US)
Pages (from-to)1318-1333
Number of pages16
JournalGCB Bioenergy
Volume11
Issue number11
DOIs
StatePublished - Nov 1 2019

Fingerprint

Chilling
C4 photosynthesis
Miscanthus
Saccharum
Photosynthesis
cold tolerance
sugarcane
photosynthesis
tolerance
Recovery
genotype
Greenhouses
Tropics
Experiments
greenhouse experimentation
experiment
introgression
rate
Miscanthus sacchariflorus
Biomass

Keywords

  • carbon dioxide partial pressure
  • chlorophyll fluorescence
  • cold tolerance
  • gas exchange
  • heritability
  • selection

ASJC Scopus subject areas

  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Waste Management and Disposal

Cite this

Saccharum × Miscanthus intergeneric hybrids (miscanes) exhibit greater chilling tolerance of C4 photosynthesis and postchilling recovery than sugarcane (Saccharum spp. hybrids). / Kar, Suraj; Zhang, Na; Nakashima, Taiken; Villanueva-Morales, Antonio; Stewart, J. Ryan; Sacks, Erik J.; Terajima, Yoshifumi; Yamada, Toshihiko.

In: GCB Bioenergy, Vol. 11, No. 11, 01.11.2019, p. 1318-1333.

Research output: Contribution to journalArticle

Kar, Suraj ; Zhang, Na ; Nakashima, Taiken ; Villanueva-Morales, Antonio ; Stewart, J. Ryan ; Sacks, Erik J. ; Terajima, Yoshifumi ; Yamada, Toshihiko. / Saccharum × Miscanthus intergeneric hybrids (miscanes) exhibit greater chilling tolerance of C4 photosynthesis and postchilling recovery than sugarcane (Saccharum spp. hybrids). In: GCB Bioenergy. 2019 ; Vol. 11, No. 11. pp. 1318-1333.
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abstract = "Although commercial sugarcane (Saccharum spp. hybrid) produces large biomass yields, its lack of cold tolerance limits its cultivation to the tropics and subtropics. In contrast, sugarcane's close relative, Miscanthus, tolerates low temperatures. We studied 18 miscane genotypes, derived from hybridizations between two genotypes of sugarcane and two genotypes of Miscanthus (one each of M. sinensis and M. sacchariflorus). In an initial greenhouse experiment on long-duration chilling stress (12–13°C day/7–9°C night), photosynthetic rates of the Miscanthus parents were significantly higher than the sugarcane parents after 7 days of chilling and were more than double by 14 days. The Miscanthus also retained more of their prechilling (22–25°C day/13–15°C night) photosynthetic rates (68{\%}–72{\%} 7 days, 64{\%}–66{\%} 14 days) than the sugarcanes (27{\%} 7 days, 19{\%}–20{\%} 14 days). Seven of 18 miscanes exhibited higher photosynthetic rates than their sugarcane parents after 7 days of chilling, whereas after 14 days only four miscane genotypes had significantly higher photosynthetic rates than their sugarcane parents, but notably two of these did not differ from their highly tolerant Miscanthus parents. In a subsequent growth chamber experiment to evaluate short-duration chilling stress and postchilling recovery, three miscanes representing the range of responses observed in the greenhouse experiment were compared with their parents. After 4 days of chilling (12/7°C day/night), the miscanes retained between 45{\%} and 60{\%} of their prechilling photosynthetic rate, with the best entry not significantly different from its Miscanthus parent (66{\%}), and all three miscanes performed significantly better than the sugarcane parents (32{\%}–33{\%} for sugarcanes). After 7 days of postchilling recovery (26/18°C day/night), the Miscanthus parents and two of the miscanes fully recovered their prechilling photosynthetic rates but the sugarcane parents only recovered 69{\%}–73{\%} of their prechilling rates. Thus, genes from Miscanthus can be used to improve chilling tolerance of sugarcane via introgression.",
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