Ruminant metabolic systems biology: Reconstruction and integration of transcriptome dynamics underlying functional responses of tissues to nutrition and physiological state

Massimo Bionaz, Juan J. Loor

Research output: Contribution to journalReview articlepeer-review

Abstract

High-throughput 'omics' data analysis via bioinformatics is one key component of the systems biology approach. The systems approach is particularly well-suited for the study of the interactions between nutrition and physiological state with tissue metabolism and functions during key life stages of organisms such as the transition from pregnancy to lactation in mammals, ie, the peripartal period. In modern dairy cows with an unprecedented genetic potential for milk synthesis, the nature of the physiologic and metabolic adaptations during the peripartal period is multifaceted and involves key tissues such as liver, adipose, and mammary. In order to understand such adaptation, we have reviewed several works performed in our and other labs. In addition, we have used a novel bioinformatics approach, Dynamic Impact Approach (DIA), in combination with partly previously published data to help interpret longitudinal biological adaptations of bovine liver, adipose, and mammary tissue to lactation using transcriptomics datasets. Use of DIA with transcriptomic data from those tissues during normal physiological adaptations and in animals fed different levels of energy prepartum allowed visualization and integration of most-impacted metabolic pathways around the time of parturition. The DIA is a suitable tool for applying the integrative systems biology approach. The ultimate goal is to visualize the complexity of the systems at study and uncover key molecular players involved in the tissue's adaptations to physiological state or nutrition.

Original languageEnglish (US)
Pages (from-to)109-125
Number of pages17
JournalGene Regulation and Systems Biology
Volume2012
Issue number6
DOIs
StatePublished - 2012

Keywords

  • Bioinformatics
  • Bovine
  • Dairy cow
  • Dynamic impact approach
  • Lactation
  • Microarray

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Computer Science Applications

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