@article{93048207efc74c8e9d89e22fbea83853,
title = "Rumen-Targeted Mining of Enzymes for Bioenergy Production",
abstract = "Second-generation biofuel production, which aims to convert lignocellulose to liquid transportation fuels, could be transformative in worldwide energy portfolios. A bottleneck impeding its large-scale deployment is conversion of the target polysaccharides in lignocellulose to their unit sugars for microbial fermentation to the desired fuels. Cellulose and hemicellulose, the two major polysaccharides in lignocellulose, are complex in nature, and their interactions with pectin and lignin further increase their recalcitrance to depolymerization. This review focuses on the intricate linkages present in the feedstocks of interest and examines the potential of the enzymes evolved by microbes, in the microbe/ruminant symbiotic relationship, to depolymerize the target polysaccharides. We further provide insights to how a rational and more efficient assembly of rumen microbial enzymes can be reconstituted for lignocellulose degradation. We conclude by expounding on how gains in this area can impact the sustainability of both animal agriculture and the energy sector.",
keywords = "bioenergy, biofuels, enzymes, lignocellulose, rumen",
author = "Isaac Cann and Yanfen Cheng and Alhawsawi, {Manal A.B.} and Mallory Moran and Yuqi Li and Tian Gong and Weiyun Zhu and Mackie, {Roderick I.}",
note = "The authors are aware that there could be significant contributions in this field that have not been cited in this review. It is our hope, however, that the review will spur interest in the research area in general and therefore the research of all colleagues in the field. Research of I.C. and R.I.M. on plant cell wall degrading enzymes was supported by the Energy Biosciences Institute and by the National Institute of General Medical Sciences of the National Institutes of Health under award no. RO1GM140306. The research of Y.C. andW.Z. on plant cell wall hydrolysis enzymes is funded by the National Science Foundation of China (32061143034, 32372905). The authors are aware that there could be significant contributions in this field that have not been cited in this review. It is our hope, however, that the review will spur interest in the research area in general and therefore the research of all colleagues in the field. Research of I.C. and R.I.M. on plant cell wall\u2013degrading enzymes was supported by the Energy Biosciences Institute and by the National Institute of General Medical Sciences of the National Institutes of Health under award no. RO1GM140306. The research of Y.C. and W.Z. on plant cell wall hydrolysis enzymes is funded by the National Science Foundation of China (32061143034, 32372905).",
year = "2025",
month = feb,
day = "18",
doi = "10.1146/annurev-Animal-021022-030040",
language = "English (US)",
volume = "13",
pages = "343--369",
journal = "Annual Review of Animal Biosciences",
issn = "2165-8102",
publisher = "Annual Reviews Inc.",
number = "1",
}