RU486 suppresses prolactin production in explant cultures of leiomyoma and myometrium

E. A. Stewart; D. J. Austin; P. Jain; M. D. Penglase; R. A. Nowak

doi:10.1016/s0015-0282(16)58324-1

RU486 suppresses prolactin production in explant cultures of leiomyoma and myometrium

E. A. Stewart, D. J. Austin, P. Jain, M. D. Penglase, R. A. Nowak

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To assess the action of RU486 (mifepristone), in the presence and absence of P, on PRL production by explant cultures of leiomyoma and myometrium. Design: Explant cultures using tissue from nine premenopausal women undergoing hysterectomy in the proliferative phase of the menstrual cycle; immunohistochemical staining of tissue sections from five patients for P receptor (PR) subtype. Main Outcome Measures: Prolactin secretion (measured by RIA), lactate dehydrogenase secretion (measured by quantitative colorimetric assay), and immunohistochemistry for PR subtype. Results: Prolactin secretion was decreased in leiomyomas by RU486 at concentrations of 10^-7 M and 10^-5 M when normal serum-containing medium was used. In experiments with all detectable P removed from serum, PRL secretion was suppressed in both leiomyomas and myometrium at an RU486 concentration of 10^-7 M. Immunohistochemistry results suggest that the A form of the PR is the dominant form in both leiomyomas and myometrium. Conclusion: Prolactin production is suppressed in both leiomyomas and myometrium after treatment with RU486 in vitro, and this suppression may serve as a marker for the clinical effectiveness of agents used in the treatment of leiomyomas.

Original language	English (US)
Pages (from-to)	1119-1124
Number of pages	6
Journal	Fertility and Sterility
Volume	65
Issue number	6
DOIs	https://doi.org/10.1016/s0015-0282(16)58324-1
State	Published - 1996
Externally published	Yes

Keywords

Mifepristone
leiomyoma
myometrium
progesterone receptor
prolactin

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

Online availability

10.1016/s0015-0282(16)58324-1

Library availability

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@article{383464809d9e44d298e4e852410bcaaf,

title = "RU486 suppresses prolactin production in explant cultures of leiomyoma and myometrium",

abstract = "Objective: To assess the action of RU486 (mifepristone), in the presence and absence of P, on PRL production by explant cultures of leiomyoma and myometrium. Design: Explant cultures using tissue from nine premenopausal women undergoing hysterectomy in the proliferative phase of the menstrual cycle; immunohistochemical staining of tissue sections from five patients for P receptor (PR) subtype. Main Outcome Measures: Prolactin secretion (measured by RIA), lactate dehydrogenase secretion (measured by quantitative colorimetric assay), and immunohistochemistry for PR subtype. Results: Prolactin secretion was decreased in leiomyomas by RU486 at concentrations of 10-7 M and 10-5 M when normal serum-containing medium was used. In experiments with all detectable P removed from serum, PRL secretion was suppressed in both leiomyomas and myometrium at an RU486 concentration of 10-7 M. Immunohistochemistry results suggest that the A form of the PR is the dominant form in both leiomyomas and myometrium. Conclusion: Prolactin production is suppressed in both leiomyomas and myometrium after treatment with RU486 in vitro, and this suppression may serve as a marker for the clinical effectiveness of agents used in the treatment of leiomyomas.",

keywords = "Mifepristone, leiomyoma, myometrium, progesterone receptor, prolactin",

author = "Stewart, {E. A.} and Austin, {D. J.} and P. Jain and Penglase, {M. D.} and Nowak, {R. A.}",

note = "Funding Information: Received August 7, 1995; revised and accepted December 18, 1995. * Supported by the Berlex Scholar Award, The Berlex Foundation, New York, New York (E.A.S.) and by HD-30496, National Institutes of Health, Bethesda, Maryland (to R.A.N.). t Presented at the Conjoint Meeting of The American Fertility Society and The Canadian Fertility and Andrology Society, Montreal, Quebec, Canada, October 11 to 14, 1993. :j: Reprint requests: Elizabeth A. Stewart, M.D., Laboratory for Human Reproduction and Reproductive Biology, Room 449, Boston Lying-In, 221 Longwood Avenue, Boston, Massachusetts 02115 (FAX: 617-566-7752).",

year = "1996",

doi = "10.1016/s0015-0282(16)58324-1",

language = "English (US)",

volume = "65",

pages = "1119--1124",

journal = "Fertility and Sterility",

issn = "0015-0282",

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TY - JOUR

T1 - RU486 suppresses prolactin production in explant cultures of leiomyoma and myometrium

AU - Stewart, E. A.

AU - Austin, D. J.

AU - Jain, P.

AU - Penglase, M. D.

AU - Nowak, R. A.

N1 - Funding Information: Received August 7, 1995; revised and accepted December 18, 1995. * Supported by the Berlex Scholar Award, The Berlex Foundation, New York, New York (E.A.S.) and by HD-30496, National Institutes of Health, Bethesda, Maryland (to R.A.N.). t Presented at the Conjoint Meeting of The American Fertility Society and The Canadian Fertility and Andrology Society, Montreal, Quebec, Canada, October 11 to 14, 1993. :j: Reprint requests: Elizabeth A. Stewart, M.D., Laboratory for Human Reproduction and Reproductive Biology, Room 449, Boston Lying-In, 221 Longwood Avenue, Boston, Massachusetts 02115 (FAX: 617-566-7752).

PY - 1996

Y1 - 1996

N2 - Objective: To assess the action of RU486 (mifepristone), in the presence and absence of P, on PRL production by explant cultures of leiomyoma and myometrium. Design: Explant cultures using tissue from nine premenopausal women undergoing hysterectomy in the proliferative phase of the menstrual cycle; immunohistochemical staining of tissue sections from five patients for P receptor (PR) subtype. Main Outcome Measures: Prolactin secretion (measured by RIA), lactate dehydrogenase secretion (measured by quantitative colorimetric assay), and immunohistochemistry for PR subtype. Results: Prolactin secretion was decreased in leiomyomas by RU486 at concentrations of 10-7 M and 10-5 M when normal serum-containing medium was used. In experiments with all detectable P removed from serum, PRL secretion was suppressed in both leiomyomas and myometrium at an RU486 concentration of 10-7 M. Immunohistochemistry results suggest that the A form of the PR is the dominant form in both leiomyomas and myometrium. Conclusion: Prolactin production is suppressed in both leiomyomas and myometrium after treatment with RU486 in vitro, and this suppression may serve as a marker for the clinical effectiveness of agents used in the treatment of leiomyomas.

AB - Objective: To assess the action of RU486 (mifepristone), in the presence and absence of P, on PRL production by explant cultures of leiomyoma and myometrium. Design: Explant cultures using tissue from nine premenopausal women undergoing hysterectomy in the proliferative phase of the menstrual cycle; immunohistochemical staining of tissue sections from five patients for P receptor (PR) subtype. Main Outcome Measures: Prolactin secretion (measured by RIA), lactate dehydrogenase secretion (measured by quantitative colorimetric assay), and immunohistochemistry for PR subtype. Results: Prolactin secretion was decreased in leiomyomas by RU486 at concentrations of 10-7 M and 10-5 M when normal serum-containing medium was used. In experiments with all detectable P removed from serum, PRL secretion was suppressed in both leiomyomas and myometrium at an RU486 concentration of 10-7 M. Immunohistochemistry results suggest that the A form of the PR is the dominant form in both leiomyomas and myometrium. Conclusion: Prolactin production is suppressed in both leiomyomas and myometrium after treatment with RU486 in vitro, and this suppression may serve as a marker for the clinical effectiveness of agents used in the treatment of leiomyomas.

KW - Mifepristone

KW - leiomyoma

KW - myometrium

KW - progesterone receptor

KW - prolactin

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DO - 10.1016/s0015-0282(16)58324-1

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SN - 0015-0282

VL - 65

SP - 1119

EP - 1124

JO - Fertility and Sterility

JF - Fertility and Sterility

IS - 6

ER -

RU486 suppresses prolactin production in explant cultures of leiomyoma and myometrium

Abstract

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