RU486 suppresses prolactin production in explant cultures of leiomyoma and myometrium

E. A. Stewart, D. J. Austin, P. Jain, M. D. Penglase, R. A. Nowak

Research output: Contribution to journalArticlepeer-review


Objective: To assess the action of RU486 (mifepristone), in the presence and absence of P, on PRL production by explant cultures of leiomyoma and myometrium. Design: Explant cultures using tissue from nine premenopausal women undergoing hysterectomy in the proliferative phase of the menstrual cycle; immunohistochemical staining of tissue sections from five patients for P receptor (PR) subtype. Main Outcome Measures: Prolactin secretion (measured by RIA), lactate dehydrogenase secretion (measured by quantitative colorimetric assay), and immunohistochemistry for PR subtype. Results: Prolactin secretion was decreased in leiomyomas by RU486 at concentrations of 10-7 M and 10-5 M when normal serum-containing medium was used. In experiments with all detectable P removed from serum, PRL secretion was suppressed in both leiomyomas and myometrium at an RU486 concentration of 10-7 M. Immunohistochemistry results suggest that the A form of the PR is the dominant form in both leiomyomas and myometrium. Conclusion: Prolactin production is suppressed in both leiomyomas and myometrium after treatment with RU486 in vitro, and this suppression may serve as a marker for the clinical effectiveness of agents used in the treatment of leiomyomas.

Original languageEnglish (US)
Pages (from-to)1119-1124
Number of pages6
JournalFertility and Sterility
Issue number6
StatePublished - 1996
Externally publishedYes


  • Mifepristone
  • leiomyoma
  • myometrium
  • progesterone receptor
  • prolactin

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


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