RT-PCR analysis of Candida albicans ALS gene expression in a hyposalivatory rat model of oral candidiasis and in HIV-positive human patients

Clayton B. Green, Sandra Manfra Marretta, Georgina Cheng, Fady F. Faddoul, E. J. Ehrhart, Lois L. Hoyer

Research output: Contribution to journalArticle

Abstract

ALS gene expression was studied in the hyposalivatory rat model of oral candidiasis and in clinical specimens collected from HIV-positive patients to assess similarities in expression patterns between the model system and clinical isolates. Two Candida albicans strains, SC5314 and OY-2-76, were used in the rat model system and infection progressed for 3 or 5 days. The strains produced similar oral lesions at 3 days. At 5 days, strain OY-2-76 produced more superficial lesions containing relatively more yeast forms compared to invasive hyphal forms observed for strain SC5314. For all infections, the most severe lesions were observed on the tongue and gingiva overlying the mandible. ALS transcripts were easier to detect by RT-PCR later in infection and under other conditions where more fungal cells were present. Expression of ALS1, ALS2, ALS3 and ALS4 was observed in rats infected for 3 days with ALS5 and ALS9 transcripts detected after 5 days of infection. Expression of ALS6 was observed in a single specimen from a 5-day infection while ALS7 transcript was never found. Expression of all ALS genes was observed in oral clinical material collected from HIV-positive patients although ALS6 and ALS7 transcripts required an extra PCR amplification step to be detected. Overall, the patterns of ALS gene expression were similar between the rat model and human clinical specimens, suggesting that the model would be useful for studying the phenotype of alδ/alδ mutant strains.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalMedical Mycology
Volume44
Issue number2
DOIs
StatePublished - Mar 2006

Keywords

  • Animal model
  • Candida
  • Gene expression
  • Gene family
  • Human immunodeficiency virus
  • Oral candidiasis

ASJC Scopus subject areas

  • Infectious Diseases

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