Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy

Matthew D. Lew; Mikael P. Backlund; W. E. Moerner

doi:10.1021/nl304359p

Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy

Matthew D. Lew, Mikael P. Backlund, W. E. Moerner

Research output: Contribution to journal › Article › peer-review

Abstract

The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle α > 60 can mislocalization errors be bounded to ≤10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.

Original language	English (US)
Pages (from-to)	3967-3972
Number of pages	6
Journal	Nano letters
Volume	13
Issue number	9
DOIs	https://doi.org/10.1021/nl304359p
State	Published - Sep 11 2013
Externally published	Yes

Keywords

dipole emission pattern
localization error
Localization microscopy
molecular orientation
rotational diffusion

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

Online availability

10.1021/nl304359p

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AB - The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle α > 60 can mislocalization errors be bounded to ≤10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.

KW - dipole emission pattern

KW - localization error

KW - Localization microscopy

KW - molecular orientation

KW - rotational diffusion

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Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy

Abstract

Keywords

ASJC Scopus subject areas

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