Rose (Rosa hybrida L.).

Schuyler S. Korban, Ksenija Gasic, Xiangqian Li

Research output: Contribution to journalArticlepeer-review

Abstract

Although rose transformation is successful, it remains difficult to transform myriad rose species as well as different rose genotypes. In this protocol, a detailed description of rose transformation is presented. This protocol relied on Agrobacterium-mediated transfer of embryogenic callus cultures. There are many critical steps that must be followed to achieve successful transformation; however, it is important to keep in mind that these apply to a selected number of genotypes, and as a different genotype is subjected to transformation, modifications of this protocol must be made to achieve successful transformation.

Original languageEnglish (US)
Pages (from-to)351-358
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Volume344
StatePublished - 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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