TY - JOUR
T1 - Rose (Rosa hybrida L.).
AU - Korban, Schuyler S.
AU - Gasic, Ksenija
AU - Li, Xiangqian
PY - 2006
Y1 - 2006
N2 - Although rose transformation is successful, it remains difficult to transform myriad rose species as well as different rose genotypes. In this protocol, a detailed description of rose transformation is presented. This protocol relied on Agrobacterium-mediated transfer of embryogenic callus cultures. There are many critical steps that must be followed to achieve successful transformation; however, it is important to keep in mind that these apply to a selected number of genotypes, and as a different genotype is subjected to transformation, modifications of this protocol must be made to achieve successful transformation.
AB - Although rose transformation is successful, it remains difficult to transform myriad rose species as well as different rose genotypes. In this protocol, a detailed description of rose transformation is presented. This protocol relied on Agrobacterium-mediated transfer of embryogenic callus cultures. There are many critical steps that must be followed to achieve successful transformation; however, it is important to keep in mind that these apply to a selected number of genotypes, and as a different genotype is subjected to transformation, modifications of this protocol must be made to achieve successful transformation.
UR - http://www.scopus.com/inward/record.url?scp=39049183257&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=39049183257&partnerID=8YFLogxK
U2 - 10.1385/1-59745-131-2:351
DO - 10.1385/1-59745-131-2:351
M3 - Article
C2 - 17033077
AN - SCOPUS:39049183257
SN - 1064-3745
VL - 344
SP - 351
EP - 358
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -