Role of the tightly bound quinone for the oxygen reaction of cytochrome bo3 oxidase from Escherichia coli

Frédéric Melin, Sinan Sabuncu, Sylvia K. Choi, Agathe Leprince, Robert B Gennis, Petra Hellwig

Research output: Contribution to journalArticle

Abstract

The coupling of the reaction of a tightly bound ubiquinone with the reduction of O2 in cytochrome bo3 of Escherichia coli was investigated. In the absence of the quinone, a strongly diminished rate of electrocatalytic reduction of oxygen is detected, which can be restored by adding quinones. The correlation of previous EPR data with the electrocatalytic study on mutations in the binding site at positions, Q101, D75, F93, H98, I102 and R71 reveal that the stabilization of the radical is not necessary for the oxygen reaction. The Q101 and F93 variants exhibit both well-defined catalytic i–V curves, whereas D75H, H98F, I102W and R71H exhibit broad i–V curves with large hysteresis pointing toward a strong alteration in their catalytic activity.

Original languageEnglish (US)
Pages (from-to)3380-3387
Number of pages8
JournalFEBS Letters
Volume592
Issue number20
DOIs
StatePublished - Oct 2018

Fingerprint

Electron Transport Complex IV
Escherichia coli
Oxygen
Quinones
Ubiquinone
Cytochromes
Paramagnetic resonance
Hysteresis
Catalyst activity
Stabilization
Binding Sites
Mutation
benzoquinone

Keywords

  • direct electron transfer
  • oxygen reduction
  • protein film voltammetry
  • quinol binding site
  • quinol oxidase
  • site-directed mutagenesis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Role of the tightly bound quinone for the oxygen reaction of cytochrome bo3 oxidase from Escherichia coli. / Melin, Frédéric; Sabuncu, Sinan; Choi, Sylvia K.; Leprince, Agathe; Gennis, Robert B; Hellwig, Petra.

In: FEBS Letters, Vol. 592, No. 20, 10.2018, p. 3380-3387.

Research output: Contribution to journalArticle

Melin, Frédéric ; Sabuncu, Sinan ; Choi, Sylvia K. ; Leprince, Agathe ; Gennis, Robert B ; Hellwig, Petra. / Role of the tightly bound quinone for the oxygen reaction of cytochrome bo3 oxidase from Escherichia coli. In: FEBS Letters. 2018 ; Vol. 592, No. 20. pp. 3380-3387.
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