Role of membrane protein Imp of “flavescence dorée” phytoplasma in interaction with insect proteins: preliminary results

Valeria Trivellone; Matteo Ripamonti; Elisa Angelini; Luisa Filippin; Marika Rossi; Cristina Marzachí; Luciana Galetto

doi:10.5958/2249-4677.2019.00061.6

Role of membrane protein Imp of “flavescence dorée” phytoplasma in interaction with insect proteins: preliminary results

Valeria Trivellone, Matteo Ripamonti, Elisa Angelini, Luisa Filippin, Marika Rossi, Cristina Marzachí, Luciana Galetto

Illinois Natural History Survey

Research output: Contribution to journal › Article › peer-review

Abstract

The involvement of the immunodominant membrane protein Imp of 16SrV-D phytoplasma in the interaction with insect proteins was investigated. The His-tagged partial Imp protein and a rabbit polyclonal antibody were produced and used in Far-Western dot Blot (FWdB) assays. Total native proteins were extracted from batches of six insect species (Scaphoideus titanus, Euscelidius variegatus, Macrosteles quadripunctulatus, Ricania speculum, Metcalfa pruinosa and Zyginidia pullula). FWdB showed interaction of Imp fusion protein with total proteins of S. titanus, E. variegatus (known as efficient vectors) and M. quadripuncltulatus (potential vector), while no interaction signal was detected with the other three species that are reported as non-phytoplasma vectors.

Original language	English (US)
Pages (from-to)	121-122
Number of pages	2
Journal	Phytopathogenic Mollicutes
Volume	9
Issue number	1
DOIs	https://doi.org/10.5958/2249-4677.2019.00061.6
State	Published - Jun 2019

Keywords

INHS
Leafhoppers
Grapevine yellows
Planthoppers
Imp

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases
Ecology, Evolution, Behavior and Systematics
Cell Biology
Parasitology

Online availability

10.5958/2249-4677.2019.00061.6

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title = "Role of membrane protein Imp of “flavescence dor{\'e}e” phytoplasma in interaction with insect proteins: preliminary results",

abstract = "The involvement of the immunodominant membrane protein Imp of 16SrV-D phytoplasma in the interaction with insect proteins was investigated. The His-tagged partial Imp protein and a rabbit polyclonal antibody were produced and used in Far-Western dot Blot (FWdB) assays. Total native proteins were extracted from batches of six insect species (Scaphoideus titanus, Euscelidius variegatus, Macrosteles quadripunctulatus, Ricania speculum, Metcalfa pruinosa and Zyginidia pullula). FWdB showed interaction of Imp fusion protein with total proteins of S. titanus, E. variegatus (known as efficient vectors) and M. quadripuncltulatus (potential vector), while no interaction signal was detected with the other three species that are reported as non-phytoplasma vectors.",

keywords = "INHS, Leafhoppers, Grapevine yellows, Planthoppers, Imp",

author = "Valeria Trivellone and Matteo Ripamonti and Elisa Angelini and Luisa Filippin and Marika Rossi and Cristina Marzach{\'i} and Luciana Galetto",

note = "Funding Information: This research was funded by the Swiss National Science Foundation (P2NEP3_168526/3) and partially by H2020, TROPICSAFE project (contract No. 727459). Publisher Copyright: {\textcopyright} 2019, Technology Society of Basic and Applied Sciences. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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doi = "10.5958/2249-4677.2019.00061.6",

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AU - Trivellone, Valeria

AU - Ripamonti, Matteo

AU - Angelini, Elisa

AU - Filippin, Luisa

AU - Rossi, Marika

AU - Marzachí, Cristina

AU - Galetto, Luciana

N1 - Funding Information: This research was funded by the Swiss National Science Foundation (P2NEP3_168526/3) and partially by H2020, TROPICSAFE project (contract No. 727459). Publisher Copyright: © 2019, Technology Society of Basic and Applied Sciences. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2019/6

Y1 - 2019/6

N2 - The involvement of the immunodominant membrane protein Imp of 16SrV-D phytoplasma in the interaction with insect proteins was investigated. The His-tagged partial Imp protein and a rabbit polyclonal antibody were produced and used in Far-Western dot Blot (FWdB) assays. Total native proteins were extracted from batches of six insect species (Scaphoideus titanus, Euscelidius variegatus, Macrosteles quadripunctulatus, Ricania speculum, Metcalfa pruinosa and Zyginidia pullula). FWdB showed interaction of Imp fusion protein with total proteins of S. titanus, E. variegatus (known as efficient vectors) and M. quadripuncltulatus (potential vector), while no interaction signal was detected with the other three species that are reported as non-phytoplasma vectors.

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Role of membrane protein Imp of “flavescence dorée” phytoplasma in interaction with insect proteins: preliminary results

Abstract

Keywords

ASJC Scopus subject areas

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