Role of aspartate 132 at the orifice of a proton pathway in cytochrome c oxidase

Ann Louise Johansson, Martin Högbom, Jens Carlsson, Robert B Gennis, Peter Brzezinski

Research output: Contribution to journalArticle

Abstract

Proton transfer across biological membranes underpins central processes in biological systems, such as energy conservation and transport of ions and molecules. In the membrane proteins involved in these processes, proton transfer takes place through specific pathways connecting the two sides of the membrane via control elements within the protein. It is commonly believed that acidic residues are required near the orifice of such proton pathways to facilitate proton uptake. In cytochrome c oxidase, one such pathway starts near a conserved Asp-132 residue. Results from earlier studies have shown that replacement of Asp-132 by, e.g., Asn, slows proton uptake by a factor of ~5,000. Here, we show that proton uptake at full speed (~104s-1) can be restored in the Asp-132-Asn oxidase upon introduction of a second structural modification further inside the pathway (Asn-139-Thr) without compensating for the loss of the negative charge. This protonuptake rate was insensitive to Zn2+ addition, which in the wildtype cytochrome c oxidase slows the reaction, indicating that Asp-132 is required for Zn2+ binding. Furthermore, in the absence of Asp-132 and with Thr at position 139, at high pH (>9), proton uptake was significantly accelerated. Thus, the data indicate that Asp-132 is not strictly required for maintaining rapid proton uptake. Furthermore, despite the rapid proton uptake in the Asn-139-Thr/ Asp-132-Asn mutant cytochrome c oxidase, proton pumping was impaired, which indicates that the segment around these residues is functionally linked to pumping.

Original languageEnglish (US)
Pages (from-to)8912-8917
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number22
DOIs
StatePublished - May 28 2013

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Electron Transport Complex IV
Aspartic Acid
Protons
Biological Phenomena
Membranes
Ion Transport
Oxidoreductases
Membrane Proteins

Keywords

  • Cytochrome aa3
  • Electron transfer
  • Metalloprotein
  • Respiration
  • Transporter

ASJC Scopus subject areas

  • General

Cite this

Role of aspartate 132 at the orifice of a proton pathway in cytochrome c oxidase. / Johansson, Ann Louise; Högbom, Martin; Carlsson, Jens; Gennis, Robert B; Brzezinski, Peter.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 22, 28.05.2013, p. 8912-8917.

Research output: Contribution to journalArticle

Johansson, Ann Louise ; Högbom, Martin ; Carlsson, Jens ; Gennis, Robert B ; Brzezinski, Peter. / Role of aspartate 132 at the orifice of a proton pathway in cytochrome c oxidase. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 22. pp. 8912-8917.
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