Robust emitter localization with enhanced harmonic analysis

Hongqiang Ma, Yang Liu

Research output: Contribution to journalArticlepeer-review


We present a non-iterative and model-free algorithm for three-dimensional (3D) single emitter localization. Our algorithm decodes the axial position and the emitter width via the ratio of the first and second Fourier harmonic. The retrieved width information is further used for dynamic extraction of the proper region of interest to robustly eliminate the outer noisy background, thus improving the localization precision over existing non-iterative algorithms. Using simulated and experimental datasets, we demonstrate that our algorithm achieves localization precision approaching the state-of-the-art iterative fitting-based methods in all three dimensions at two orders of magnitude faster speed, applicable in various 3D single-molecule localization techniques.

Original languageEnglish (US)
Pages (from-to)5798-5801
Number of pages4
JournalOptics Letters
Issue number23
StatePublished - Dec 1 2021
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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